164,577 results

1. pSH-EFIRES-P-AtAFB2-mCherry

   Plasmid

   #129716

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ancBE4-SpRY

   Plasmid

   #226855

   Purpose

   Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ancBE4max-SpRY
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IZ-ANXA7-mRuby3

   Plasmid

   #184893

   Purpose

   Stable expression of ANXA7-mRuby3 (Annexin A7-mRuby3) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

   Insert

   ANXA7 (ANXA7 Human)
   

   Use

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

   Plasmid

   #140892

   Purpose

   Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.

   Depositor

   Yasushi Okamura

   Article

   Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

   Insert

   CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed 223L to F in Dr-VSP (zebrafish TPTE)

   Promoter

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVH-EF1a-GFP-P2A-MYC

   Plasmid

   #130695

   Purpose

   Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human MYC (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST47-SAR1-GFP

   Plasmid

   #67409

   Purpose

   Mammalian expression of hSar1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SAR1 (SAR1A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pUGI-NLS

   Plasmid

   #101091

   Purpose

   Expresses UGI in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

   Insert

   UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST/N1-hBIN1-GFP

   Plasmid

   #27305

   Depositor

   Robin Shaw

   Article

   Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

   Insert

   bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPAmCherry1-C1

   Plasmid

   #31929

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNitET-SacB-kan

    Plasmid

    #107692

    Purpose

    Expression of the phage Che9c RecET system for Recombineering in mycobacteria

    Depositor

    Kenan Murphy

    Article

    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

    Inserts

    Che9 phage RecET
    PNit2-NitR
    SacRB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Synapsin-pm-iATPSnFR1.0

    Plasmid

    #102555

    Purpose

    expresses membrane displayed ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204189

    Purpose

    Doxycycline inducible lentiviral vector for human GATA6 overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    GATA6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-tetO-Cebpa

    Plasmid

    #139834

    Purpose

    doxycycline-inducible expression of mouse Cebpa in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Cebpa (Cebpa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. ASAP2f

    Plasmid

    #83960

    Purpose

    Fluorescent reporter for voltage imaging

    Depositor

    Michael Lin

    Article

    Yang et al Cell. 2016 Jun 30;166(1):245-57. doi: 10

    Insert

    ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGolden-AAV

    Plasmid

    #51424

    Purpose

    Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    lacZ
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Use

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMax_mPlum

    Plasmid

    #177830

    Purpose

    Host Cell Reactivation (HCR) control mPlum plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-eTLR

    Plasmid

    #214737

    Purpose

    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    AAVS1-eTLR (PPP1R12C CMV, Anguilla japonica, influenza, Human, Bovine, Synthetic, Rabbit, Chicken)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWM_12x601_45bpLinker

    Plasmid

    #157791

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_45bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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