We narrowed to 15,037 results for: pET

1. HM3C NaVAe1_p pET24b

   Plasmid

   #127854

   Purpose

   Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVAe1p
   

   Use

   Tags

   3C Cleavage site, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 883 pET15b PTEN

   Plasmid

   #10936

   Depositor

   William Sellers

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pET-15b-LanCL1

   Plasmid

   #154189

   Purpose

   To express LanCL1 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET24a-ncOGT-TPR

   Plasmid

   #190822

   Purpose

   For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   C467X. Also codon optimized for expression in E.c…

   Promoter

   T7

   Available Since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET28-At_PRORP2-His

   Plasmid

   #67870

   Purpose

   bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP2 (PRORP2 Mustard Weed)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Pet15b-Internalization Control

   Plasmid

   #186553

   Purpose

   Negative Control mediating endosomal localization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-EGFP
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 His10-FcRɣ

   Plasmid

   #113021

   Purpose

   for purification of His10-FcRɣ intracellular domain

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   His10-FcRɣ intracellular domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b-mLin28A-cHIS

   Plasmid

   #137998

   Purpose

   Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cells

   Depositor

   Judy Lieberman

   Article

   O'Day et al J Biol Chem. 2015 Jul 17;290(29):17909-2

   Insert

   mouse Lin28A, CSD and ZnFs (residues 36–186)
   

   Use

   Tags

   C-terminal His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-NCBD + ACTR

    Plasmid

    #99335

    Purpose

    Bacterial coexpression of CBP NCBD and NCoA3 binding domains

    Depositor

    Peter Wright

    Article

    Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

    Inserts

    CBP NCBD (2059-2117) (CREBBP Mouse)
    SRC3 (1023-1093) (NCOA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET22b-BirA-CPDSalI

    Plasmid

    #38258

    Depositor

    Aimee Shen, Matthew Bogyo

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Insert

    birA (birA E. coli)
    

    Use

    Tags

    6xHis and Vibrio cholerae MARTX toxin cysteine pr…

    Expression

    Bacterial

    Mutation

    cloned from pGEX4T1-BirA

    Promoter

    T7

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET-32a(+)-TGFB3m

    Plasmid

    #120289

    Purpose

    Produce human recombinant mTGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    monomeric Transforming Growth Factor-β3
    

    Use

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-51b(+) ChemoG1

    Plasmid

    #193799

    Purpose

    Protein production of ChemoG1 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG1
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-51b(+) ChemoG2

    Plasmid

    #193800

    Purpose

    Protein production of ChemoG2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP: A206K

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-51b(+) ChemoG3

    Plasmid

    #193801

    Purpose

    Protein production of ChemoG3 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG3
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K; HT7: L271E

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-51b(+) ChemoG4

    Plasmid

    #193804

    Purpose

    Protein production of ChemoG4 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG4
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K; HT7: E143R, E147R, L271E

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24b-6His-fSNAP

    Plasmid

    #106999

    Purpose

    To express 6His-fSNAP protein in E.coli

    Depositor

    Melissa Moore

    Article

    Haraszti et al Methods Mol Biol. 2020;2113:17-22. doi:

    Insert

    fSNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pET29b-EPN01*-His

    Plasmid

    #84509

    Purpose

    E.coli version of EPN01*

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN01*-His_pET29b
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28b-Ets1-ETS

    Plasmid

    #85735

    Purpose

    Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

    Depositor

    Gregory Poon

    Article

    Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

    Insert

    DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
    

    Use

    Tags

    6xHis following thrombin cleavage site LVPR|G

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PET.SUMO eIF4G1 1-200

    Plasmid

    #158791

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (codon optimized)
    

    Use

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24a-LaM4-3xTS

    Plasmid

    #162777

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, HA, His6, T7, and TS site from proCCK

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CBP-HAT-pET

    Plasmid

    #21091

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    HAT domain aa 1171-1621

    Promoter

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pET-22b(+)-HpENR

    Plasmid

    #12651

    Depositor

    SeWon Suh

    Article

    Lee et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    noyl-acyl carrier protein reductase (fabI Helicobacter pylori)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-CVm-PTEN

    Plasmid

    #168032

    Purpose

    Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-PTEN(M265-N329)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 5787 pET28aSX104B/ pES42

    Plasmid

    #1229

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pET29b-ClbPpep-CHis

    Plasmid

    #48243

    Purpose

    First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP-pep
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    First 375 amino acids of the peptidase, which con…

    Promoter

    T7

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET11a-dH3-dH4

    Plasmid

    #105502

    Purpose

    Co-expression of Drosophila histones H3 and H4

    Depositor

    James Kadonaga

    Article

    Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

    Insert

    dH3-dH4
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge