We narrowed to 4,045 results for: golden gate
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Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS1528
Plasmid#205982PurposeU6-MWEE01000013 Cas5-HNH DR-BpiI-DRDepositorInsertCas5-HNH DR-BpiI-DR
ExpressionMammalianPromoterU6Available SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-RiboJ-LacZa-T7
Plasmid#173224PurposepFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systemsDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceMarch 9, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC71167
Plasmid#154066PurposeLevel 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFPDepositorInsertZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
UseSynthetic BiologyMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterZea mays Ubiquitin PromoterAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK403
Plasmid#110599PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBTK401
Plasmid#110597PurposeBTK Type 8 Bacterial origin (broad-host-range) for golden gate assemblyDepositorInsertmRFP dropout (RSF1010 origin)
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOPS0385
Plasmid#133235PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0386
Plasmid#133236PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0383
Plasmid#133233PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), sfGFP (pOGG037) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-N
Plasmid#174556PurposeChloroplast-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
TagsHAExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-C
Plasmid#174557PurposeChloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
TagsFLAGExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2
Plasmid#182354PurposeNuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2
Plasmid#182356PurposeNuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)DepositorTypeEmpty backboneExpressionWormAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-3
Plasmid#126058PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMiNGo11-7
Plasmid#126057PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cellDepositorInsertsmitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL11061
Plasmid#68255PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-1
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-DIAL-YB_TATA)-mGL-2A-mCherry-BGH_pKG2848
Plasmid#246341Purpose203-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern and mCherry in the presence of ZFa and editable by Cre recombinaseDepositorInsertmGreenLantern-2A-mCherry
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(203-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3015
Plasmid#246358Purpose203-bp TET-DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(90-bp-TET-DIAL-YB-TATA-VloxP)-mGL-bGH_pKG3017
Plasmid#246360Purpose90-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by VCre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(380-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3262
Plasmid#246361Purpose380-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
(610-bp-TET-DIAL-YB-TATA)-mGL-bGH_pKG3263
Plasmid#246362Purpose610-bp DIAL Reporter Plasmid with YB_TATA expressing mGreenLantern in the presence of DOX and rtTA and editable by Cre recombinaseDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-UBF
Plasmid#247350PurposeExpresses SpCas9 and a sgRNA targeting the human UBF loci for knock-in.DepositorAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_H1-g3
Plasmid#247169PurposeU6 driven gRNA expression of H1-g3 for targeting Dn29 attH1DepositorInsertH1-g3
ExpressionMammalianPromoterU6Available SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4276 hproTGFb1 in pcDNA backbone
Plasmid#244183PurposeConstitutive expression of human pro-TGF-beta1 (with its signal sequence)DepositorInsertpro-TGF-beta1 (TGFB1 Synthetic, Human)
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4869 hVEGFA-121 in pcDNA backbone
Plasmid#244180PurposeConstitutive expression of human VEGFA-121 isoformDepositorAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4868 hVEGFA-165 in pcDNA backbone
Plasmid#244179PurposeConstitutive expression of human VEGFA-165 isoformDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4300 hIL10 in pcDNA backbone
Plasmid#244185PurposeConstitutive expression of human IL-10 (with its signal sequence)DepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1012
Plasmid#239934PurposeCsy4 as Level-0 part (Golden Gate cloning)DepositorInsertCsy4
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMTKM32
Plasmid#233504PurposeLevel 0 plasmid for MoClo, encodes inducible PGK1-tetO2p for K. marxianusDepositorInsertPGK1-tetO2p
UseSynthetic BiologyExpressionYeastMutationaddition of two tetracycline operator sequenecesAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA
Plasmid#235326PurposePiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2DepositorInsertmRuby2
UseSynthetic Biology; PiggybacExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235318PurposeLentiviral expression of mRuby2 with 3' microRNA onlyDepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA
Plasmid#235327PurposePiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2DepositorInsertmRuby2
UseSynthetic Biology; PiggybacExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235268PurposeLentiviral expression of ComMAND base gene regulating mRuby2DepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE
Plasmid#235317PurposeLentiviral expression of mRuby2 with intronic microRNA onlyDepositorInsertmRuby2
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterTRE3G (3rd-generation Tet system)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_3_multieditdropout
Plasmid#233458PurposeLevel 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniaeDepositorInsertLevel 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4
UseCRISPRAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-B/c
Plasmid#227963PurposeExpression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertB/c
ExpressionPlantPromoter35SAvailable SinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIE324-ZF6x12-C DsRed-Express2 in TUPV1
Plasmid#201536PurposeDsRed-Express2 reporter regulated by ZF6 synTF promoter (12x compact binding sites) in TUPV1 for mMoClo golden gate-based assemblyDepositorInsertDsRed-Express2
ExpressionMammalianPromoterZF6x12(C)_YB_TATA Minimal PromoterAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD1178-LPDV promoterless PuroR-P2A-miRFP720
Plasmid#201537PurposeLanding pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 geneDepositorInsertPromoterless PuroR-P2A-miRFP720
ExpressionMammalianPromoterNoneAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mOrange2
Plasmid#228608PurposeMammalian expression of mOrange2, for MuSIC barcode constructionDepositorInsertmOrange2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mBeRFP
Plasmid#228601PurposeMammalian expression of mBeRFP, for MuSIC barcode constructionDepositorInsertmBeRFP
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mT-Sapphire
Plasmid#228597PurposeMammalian expression of mT-Sapphire, for MuSIC barcode constructionDepositorInsertmT-Sapphire
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-LSSmOrange
Plasmid#228600PurposeMammalian expression of LSSmOrange, for MuSIC barcode constructionDepositorInsertLSSmOrange
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTFP1
Plasmid#228602PurposeMammalian expression of mTFP1, for MuSIC barcode constructionDepositorInsertmTFP1
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mT-Sapphire
Plasmid#228616PurposeMammalian expression of mT-Sapphire and extra insert, for MuSIC barcode constructionDepositorInsertmT-Sapphire
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-miRFP670-TRF1
Plasmid#227378PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, WT (TERF1 Human)
UseLentiviralAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only