We narrowed to 2,343 results for: dcas9

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pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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ampl43

Plasmid

#161830

Purpose

pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

Depositor

Saeed Tavazoie

Article

Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

TEF1

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-MCS-Ds

Plasmid

#102416

Purpose

Empty vector with a multiple cloning site flanked by Ds integration elements

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGH156_MS2-AID-Hygro

Plasmid

#85415

Purpose

lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHelper-CRISPRi-sgRNA

Plasmid

#221135

Purpose

Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with dCas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGH153_MS2-AIDΔ-Hygro

Plasmid

#85416

Purpose

lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRnc-gGFP161

Plasmid

#189536

Purpose

Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

Depositor

Marc Ostermeier

Article

Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

Inserts

RNase III
gUTR-161-GFP

Expression

Bacterial

Mutation

N/A

Promoter

J23119 and pBAD

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGH183_MS2-AIDΔDead-Hygro

Plasmid

#85414

Purpose

lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AIDΔDead and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA221rAPOBEC1-T7RNAP

Plasmid

#167979

Purpose

Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

rAPOBEC1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-boxB-MS2-PP7

Plasmid

#75397

Purpose

sgRNA1-boxB-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

Plasmid

#84248

Purpose

Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pCas2C

Plasmid

#82374

Purpose

dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

dCas9

Promoter

PEZ3

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSL690

Plasmid

#47753

Purpose

Expresses dCas9-VP64 fusion

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Tags

3x FLAG, NLS, and VP64

Expression

Mammalian

Mutation

D10A and H840A mutations in Cas9

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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