We narrowed to 2,601 results for: ubiquitin

1. pCDFNKI-his-3C-LIC-strep

   Plasmid

   #108705

   Purpose

   Expression of a His-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   His-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN mCherry-p62(W340A/ΔUBD)

   Plasmid

   #119688

   Purpose

   For mammalian expression of LIR mutant p62 without its UBD

   Depositor

   Michael Lazarou

   Article

   Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

   Insert

   p62 (SQSTM1 Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   W340A & UBD deletion (1-385)

   Promoter

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-ubb-Avi-Cerulean-Rpl10a

   Plasmid

   #79882

   Purpose

   Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-RNF8-N-terminus(1-111)

   Plasmid

   #64671

   Purpose

   Expresses only N terminus of RNF8, amino acids 1 to 111

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   ring finger protein 8 (RNF8 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   containing only N-terminus amino acids 1 to 111

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pOPINK-OTUD1 (OTU, aa 287-437)

   Plasmid

   #61407

   Purpose

   Expresses human OTUD1 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286 and aa 438-481.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-UBE3BΔIQ-HA-IRES-Puro

   Plasmid

   #107350

   Purpose

   Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3BdelIQ (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEX-6P1-Ataxin3-1-182

   Plasmid

   #22115

   Depositor

   Henry Paulson

   Article

   Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 183-373 deleted

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pGW1-Myc-DJ1-L166P/K130R

   Plasmid

   #29349

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Leu166Pro and Lys130Arg

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1/GS-DJ1-L166P-V5

   Plasmid

   #29343

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Use

   Tags

   His6 and V5 epitope

   Expression

   Mammalian

   Mutation

   L166P

   Promoter

   Available since

   March 7, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pBW_0001

    Plasmid

    #102833

    Purpose

    Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    Sr22 Schomburgk (NEWENTRY Synthetic)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-RNF8-C-terminus

    Plasmid

    #64673

    Purpose

    expresses only C terminus of RNF8, amino acids 402-485

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    containing only C-terminus amino acids 402 to 485

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pETNKI-strepII-3C-LIC-kan

    Plasmid

    #108706

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3F-Strep-hMIB2

    Plasmid

    #33313

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Use

    Tags

    3xFlag and StrepOne

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-6P1-Ataxin3Q22-C14A

    Plasmid

    #22113

    Depositor

    Henry Paulson

    Article

    Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C14A

    Promoter

    Available since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xFLAG-CMV-RNF8-N-terminus(1-140)

    Plasmid

    #64675

    Purpose

    expresses only N terminus of RNF8, amino acids 1 to 140

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    only N terminus amino acids 1-140

    Promoter

    CMV

    Available since

    May 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid

    #79881

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-RNF8-Middle-part

    Plasmid

    #64672

    Purpose

    Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Containing only middle part of RNF8 amino acids 1…

    Promoter

    CMV

    Available since

    May 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pOPINK-OTUD6A (OTU, aa 128-288)

    Plasmid

    #61417

    Purpose

    Expresses human OTUD6A (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-127.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBacPak-HA-E(y)2

    Plasmid

    #52288

    Purpose

    Expresses Drosophila E(y)2

    Depositor

    Jerry Workman

    Article

    Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

    Insert

    E(y)2 (e(y)2 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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