2,339 results
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Plasmid#145804Purposeexpresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cellsDepositorInsertrat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
ExpressionMammalianMutationchanged serine 767 to alanine to prevent glycosyl…PromoterCMVAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr2
Plasmid#137798PurposeVisualization of beta-arrestin2DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-RnBarr1
Plasmid#137795PurposeVisualization of beta-arrestin2DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR DRY->AAY-p2A-mCherry
Plasmid#137784PurposeVisualization of the At1aR DRY mutantDepositorInsertRat At1a receptor; DRY mutant (Agtr1a Rat)
ExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
RnAT1aR DRY->AAY-mCherry
Plasmid#137783PurposeVisualization of the At1aR DRY mutantDepositorInsertRat At1a receptor; DRY mutant (Agtr1a Rat)
ExpressionMammalianMutationDRY -> AAYPromoterCMVAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_015
Plasmid#123796PurposeEncodes rat Connexin 26 as a Type 3b part to be used in the MTK systemDepositorInsertCx 26 (rat)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_017
Plasmid#123798PurposeEncodes rat Connexin 43 as a Type 3b part to be used in the MTK systemDepositorInsertCx43 (rat)
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
TagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
TagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-P22S-EGFP
Plasmid#132594PurposeExpresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorAvailable SinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ rat MYO1D
Plasmid#118419PurposeFor mammalian expression of rat myosin 1DDepositorInsertrat myosin 1D (Myo1d Rat)
ExpressionMammalianAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>Fab-srt-his
Plasmid#124810PurposeHDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.DepositorInsertrat IgG2a Hinge-G4S-Sortag-Histag
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterialPromoterpUCAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73AGW (F2)
Plasmid#74039Purposelentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationS73APromoterH1Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95Q15AD45_64GW (D8)
Plasmid#74031Purposelentiviral expression of Psd95 shRNA and Psd95-Q15A _45_64-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationQ15A, D45_64PromoterH1Available SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95L460pGW (E2)
Plasmid#74032Purposelentiviral expression of Psd95 shRNA and Psd95-L460P-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationL460PPromoterH1Available SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUP95Q15AL460PGW (E3)
Plasmid#74033Purposelentiviral expression of Psd95-Q15A L460P-EGFP fusionDepositorInsertPsd95
UseLentiviralExpressionMammalianMutationQ15A, L460PPromoterhUbCAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95DSKIrGW (D2)
Plasmid#74025Purposelentiviral expression of Psd95 shRNA and Psd95dSK and EGFPDepositorInsertPsd95
UseLentiviral and RNAiExpressionMammalianMutationdelta SKPromoterH1Available SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUP95DPESTGW (C7)
Plasmid#74023Purposelentiviral expression of Psd95-DPEST-EGFP fusionDepositorInsertPsd95
UseLentiviralExpressionMammalianMutationDPEST; amino acids 10-25 deletedPromoterhUbCAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rescue myc-PCDHGC5 mRNA
Plasmid#122229PurposeRescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
Tags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationSilent mutations are in five consecutive codons …Available SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
Plasmid#122450PurposeExpression of inositol 1,4,5-trisphosphate 3-kinase ADepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
Tagsbeta-galactosidaseExpressionBacterialMutationcoding sequence from V66 till stop codonAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3/gamma2SIL
Plasmid#121433PurposeGABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1/gamma2SIL
Plasmid#121432PurposeGABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminusDepositorTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDHGC5 sh1 shRNA
Plasmid#122227PurposeKnock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).DepositorInsertPCDHGC5 shRNA (Pcdhgc5 Rat)
UseRNAiAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDHGC5 sh1 3m shRNA
Plasmid#122228PurposeNegative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.DepositorInsertPCDHGC5 shRNA (Pcdhgc5 Rat)
UseRNAiAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1079 - pAAV TH gRNA A EF1a EGFP
Plasmid#113159PurposeAn AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporterDepositorInsertgRNA for rat TH
UseAAV and CRISPRExpressionMammalianPromotermU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP
Plasmid#113155PurposeAn AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporterDepositorInsertTwo gRNAs for rat TH
UseAAV and CRISPRExpressionMammalianPromotermU6 and hU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP
Plasmid#113157PurposeAn AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporterDepositorInsertTwo gRNAs for rat MANF
UseAAV and CRISPRExpressionMammalianPromotermU6 and hU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A
Plasmid#122155Purposerat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)DepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
ExpressionBacterialAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only