We narrowed to 8,358 results for: BLI;

1. HT7-mTurquoise2-KDEL

   Plasmid

   #188571

   Purpose

   Expresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.

   Depositor

   Pablo Rivera-Fuentes

   Insert

   HaloTag7
   

   Use

   Tags

   KDEL and mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 myc-PITPalpha

   Plasmid

   #58277

   Purpose

   mammalian expression of human PITP alpha

   Depositor

   Shamshad Cockcroft

   Insert

   PITP alpha (PITPNA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Flag-TRIM24-F869D

   Plasmid

   #28141

   Depositor

   Michelle Barton

   Insert

   TRIM24 (TRIM24 Human)
   

   Use

   Tags

   FLAGX2

   Expression

   Mammalian

   Mutation

   A489V, F869D; aa 308 and 309 mutated for shRNA re…

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET21a(+)_C.jejuni m23 peptidase

   Plasmid

   #83297

   Purpose

   Expresses m23 peptidase from Campylobacter jejuni

   Depositor

   SeWon Suh

   Insert

   ERS445056_01343
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   deleted amino acid 1-20

   Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-PE2-pegRNA-tetra-com vector

   Plasmid

   #136271

   Purpose

   Expresses PE2 and Tetra-com modified pegRNA cassette

   Depositor

   Baisong Lu

   Insert

   PE2 and pegRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p2Bac.Myc.p44

   Plasmid

   #39220

   Depositor

   Aziz Sancar

   Insert

   p44 (GTF2H2 )
   

   Use

   Tags

   Myc

   Expression

   Insect

   Mutation

   Promoter

   Pp10

   Available since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pNB3033

   Plasmid

   #113397

   Purpose

   Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible

   Depositor

   Suzanne Sandmeyer

   Insert

   Ty3 Gag3 codon optimized for E. coli expression
   

   Use

   Tags

   Gag3-6XHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

   Plasmid

   #171938

   Purpose

   Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins

   Depositor

   David Berson

   Insert

   Apex2, mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmr101A-cPr-mCherry (ErmR)

   Plasmid

   #177206

   Purpose

   Plasmid with p15A origin for constitutive mCherry expression in E. coli

   Depositor

   Alexey Merz

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Synthetic constitutive promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBS-L1PA1CH blast

    Plasmid

    #69612

    Purpose

    Expresses a blasticidin tagged codon optimized L1PA1

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA1
    

    Use

    Tags

    blast cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 4IJD

    Plasmid

    #51328

    Depositor

    Cheryl Arrowsmith

    Insert

    4IJD (PRDM9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pIC79

    Plasmid

    #63063

    Purpose

    Mammalian expression of EGFP-TEV-S-tag hNSL1 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    hNSL1, IMAGE clone 3997280 (NSL1 Human)
    

    Use

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid

    #61850

    Purpose

    cell cycle marker

    Depositor

    Jonathon Pines

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    NLS and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCAGGS-S-B.1.1.529-FLAG

    Plasmid

    #180774

    Purpose

    B.1.1.529 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein B.1.1.529
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLL1270

    Plasmid

    #129281

    Purpose

    The vector used for enzyme expression in Thermoanaerobacterium. thermosaccharolyticum throughout this study. Contains the CBP promoter from Clostridium thermocellum

    Depositor

    Lee Lynd

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLIX403-EYFP-ccdB-G418

    Plasmid

    #158550

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pORTMAGE502B

    Plasmid

    #128971

    Purpose

    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xyl-Pm

    Available since

    Aug. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK647

    Plasmid

    #71701

    Purpose

    Produces E. coli thioredoxin reductase (EcTrxB)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TBRII-C-18

    Plasmid

    #62797

    Purpose

    Localization: Membrane Receptor, C1 Cloning Vector, Excitation:N/A, Emission:N/A

    Depositor

    Michael Davidson

    Insert

    TBRII (TGFBR2 Human)
    

    Use

    Tags

    SNAP Tag

    Expression

    Mammalian

    Mutation

    H385N

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pHH0103 NEDD4L WW domain #3

    Plasmid

    #104206

    Purpose

    Bacterial expression of WW domain #3 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #3 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDSAY-vasaCas9sv40

    Plasmid

    #173669

    Purpose

    Cas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Insert

    Cas9 under control of vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcB6-Myc-Mfn2 IYFFT

    Plasmid

    #191953

    Purpose

    Expression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)

    Depositor

    Luca Scorrano

    Insert

    Myc-Mfn2-IYFFT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C- terminus replaced by the stretch of hydrophobi…

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS-L1PA13ACHmneo

    Plasmid

    #69613

    Purpose

    Expresses a neomycin tagged codon optimized L1PA13A

    Depositor

    Astrid Roy-Engel

    Insert

    L1PA13A
    

    Use

    Tags

    mneo retrotransposition cassette

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pExp-DsbA(SS)

    Plasmid

    #112563

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbA (leaderless thiol:disulfide interchange protein DsbA from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-DsbA(SS) and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid

    #72258

    Purpose

    Express FLPe, copGFP and Puromycin resistance gene under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-copGFp-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pSil-XIAP

    Plasmid

    #58832

    Purpose

    Express human apoptosis inhibitor XIAP from CAG promoter

    Depositor

    Troy Margrie

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mPA-GFP-Peroxisomes-2

    Plasmid

    #54628

    Purpose

    Localization: Peroxisomes, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Peroximal Targeting Signal 1 (PTS1) and mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. N1-LOC8R

    Plasmid

    #66896

    Purpose

    Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial targeting sequence compatible with any N1 vector. Internal restriction sites enable modular subcloning of 1x, 2x, 3x or 4x presequences.

    Depositor

    Georg Ramm

    Insert

    LOC8R
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid

    #171937

    Purpose

    Expresses APEX2 linked to tau for labeling microtubules

    Depositor

    David Berson

    Insert

    tau-APEX2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK408

    Plasmid

    #72422

    Purpose

    Produces Acetobacter aceti 1023 Est10, C-terminal His5 tag

    Depositor

    T. J. Kappock

    Insert

    feruloyl esterase
    

    Use

    Tags

    His5

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBS SK(+)/mGATA2

    Plasmid

    #83823

    Purpose

    mGATA2 cDNA

    Depositor

    Douglas Engel

    Insert

    GATA2 (Gata2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pET StrepII TEV cloning vector with BioBrick polycistronic restriction sites (9R)

    Plasmid

    #48290

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HA-tagged Abr

    Plasmid

    #38190

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pExp-Bla

    Plasmid

    #112561

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-Bla and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. RV-Syn-Dio-mVenus

    Plasmid

    #153204

    Purpose

    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter

    Depositor

    Lynette Lim

    Insert

    membrane-tagged-Venus
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    rat synapsin

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV Tmx1-V5

    Plasmid

    #175138

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx1

    Depositor

    Larry Gerace

    Insert

    Tmx1 (Tmx1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV Tmem9b-V5

    Plasmid

    #175152

    Purpose

    Lentiviral expression of mouse Tmem9b-V5

    Depositor

    Larry Gerace

    Insert

    Tmem9b (Tmem9b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHH0103 NEDD4L WW domain #1

    Plasmid

    #104204

    Purpose

    Bacterial expression of WW domain #1 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #1 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET StrepII TEV expression vector with BioBrick polypromoter restriction sites (14-R)

    Plasmid

    #48312

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pB6-Hipp11-DTA-Capecchi-rev

    Plasmid

    #127054

    Purpose

    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, reverse cloning sites, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge