We narrowed to 11,913 results for: pcDNA

1. pcDNA3-sACE2v6-sfGFP

   Plasmid

   #145177

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 myc-PITPalpha

   Plasmid

   #58277

   Purpose

   mammalian expression of human PITP alpha

   Depositor

   Shamshad Cockcroft

   Insert

   PITP alpha (PITPNA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Mouse NCKX3 (pcDNA3.1+)

   Plasmid

   #75204

   Purpose

   Mammalian expression of SLC24A3

   Depositor

   Jonathan Lytton

   Article

   Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

   Insert

   SLC24A3 (Slc24a3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Akt-STOPS

   Plasmid

   #175006

   Purpose

   Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-FRT-TO-FH-Nsp5

   Plasmid

   #157686

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp5 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3.1-ROR1-V5/HIS

   Plasmid

   #205607

   Purpose

   Expression of human ROR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR1 receptor tyrosine kinase (ROR1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3_N-DYK_MTHFD1-NES

   Plasmid

   #133022

   Purpose

   NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

   Depositor

   Stefan Kubicek

   Article

   Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

   Insert

   MTHFD1 (MTHFD1 Human)
   

   Use

   Tags

   NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)-ExRai-AKAR(T/A)

   Plasmid

   #118406

   Purpose

   Negative-control mutant for ExRai-AKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR(T/A)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA4.TO-ORF63-2xCSTREP

   Plasmid

   #136223

   Purpose

   Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF63
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-neo-Strep_Flag_Nterm

    Plasmid

    #102644

    Purpose

    Epitope tag n-terminus with SBP-Flag moiety

    Depositor

    Michael E. Wright

    Article

    Hsiao et al J Biol Chem. 2016 Sep 2;291(36):18818-42

    Type

    Empty backbone

    Use

    Tags

    SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-aDTX-lumitoxin

    Plasmid

    #51686

    Purpose

    Expression vector for aDTX-lumitoxin

    Depositor

    Edward Boyden

    Article

    Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

    Insert

    aDTX-lumitoxin
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-FBL6 delta6

    Plasmid

    #19925

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    FBL6 delta 6 (FBXL6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    delta 6

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid

    #104353

    Purpose

    Expresses human beta 2 integrin tension sensor

    Depositor

    Timothy Springer, Pontus Nordenfelt

    Article

    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

    Insert

    Human integrin b2 with tension sensor (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    AA1-733 ITGB2 present

    Promoter

    CMV

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5/FRT/TO V5 DNAJB4

    Plasmid

    #19526

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 4 (DNAJB4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)_Granulin 2+linker3

    Plasmid

    #176920

    Purpose

    express Granulin 2 with linker 3 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 2+linker3 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-sACE2v2.4(732)

    Plasmid

    #154100

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT-TO-FH-Orf6

    Plasmid

    #157727

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Orf6 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Orf6 (ORF6 SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCDNA4.TO-K8a-2xCSTREP

    Plasmid

    #136158

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K8a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA 3.1 (+) Tacδ

    Plasmid

    #81177

    Purpose

    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    Tacδ
    

    Use

    Tags

    KLGFFKR

    Expression

    Mammalian

    Mutation

    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2v1-sfGFP

    Plasmid

    #145172

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA-h-mmPtch1-FL-TagBFP

    Plasmid

    #120921

    Purpose

    Expresses BFP tagged Ptch1

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-mCherry-TSC2(AA,3Q)

    Plasmid

    #192444

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-mCherry-TSC2(AA)

    Plasmid

    #192442

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A) (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. T7-p73DD L371P-pcDNA3

    Plasmid

    #23049

    Depositor

    William Kaelin

    Article

    Irwin et al Nature. 2000 Oct 5. 407(6804):645-8.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    truncated cDNA encodes truncated protein (aa 327-…

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

    Plasmid

    #159132

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Use

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11F

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA-FRT-FAK0

    Plasmid

    #45047

    Purpose

    FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 3534 pcDNA3 Bax

    Plasmid

    #8783

    Depositor

    Stanley Korsmeyer

    Article

    Oltvai et al Cell 1993 Aug 27;74(4):609-19.

    Insert

    Bax (Bax Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

30. pCDNA4.TO-ORF39-2xCSTREP

    Plasmid

    #136200

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF39
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1(+)-TTF-1-HDD

    Plasmid

    #52063

    Purpose

    It encodes a mutant of TTF-1 in which the homeodomain is deleted

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    Thyroid Transcription Factor 1 (NKX2-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Homeodomain deleted

    Promoter

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1-CHRFAM7A-mGFP

    Plasmid

    #62633

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA3 Flag MKK4beta

    Plasmid

    #15517

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    pCDNA3 Flag MKK4beta (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pCDNA-FRT-FAK20

    Plasmid

    #59120

    Purpose

    FAK fret sensor with 20 nm helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3-HA-RIPK3 4KR

    Plasmid

    #78813

    Purpose

    To overexpress RIPK3 4KR in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1+_LN-HA-AGO2-WT

    Plasmid

    #92009

    Purpose

    Expresses Lambda-N-HA-AGO2 (WT)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-CARMA2sh-E138A

    Plasmid

    #97068

    Purpose

    Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamic Acid 138 to Alanine

    Promoter

    CMV

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-Flag-YOD1

    Plasmid

    #49784

    Purpose

    Express YOD1 with a N-terminal Flag-tag in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    Flag-tag

    Expression

    Mutation

    WT

    Promoter

    Available since

    Dec. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA5FRT/TO-p97wt-mycStrep

    Plasmid

    #31837

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Use

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only