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Showing: 3551 - 3600 of 18940 results

  1. pLP-PIGA3 (pLP305)

    Plasmid
    #196992
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196992-plasmid-map-sequence-id-389805

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  3. pMMACE DEST CMV SpCas9

    Plasmid
    #206268
    Purpose
    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9
    Use
    CRISPR; Recombinant baculovirus production
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206268-plasmid-map-sequence-id-406404

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  4. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

    Plasmid
    #50834
    Purpose
    An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.
    Depositor
    Brandon Harvey
    Article
    McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do
    Insert
    hChR2 (H134R)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    EF1a
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    50834-plasmid-map-sequence-id-76270

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  7. pJW1927

    Plasmid
    #163093
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    mScarlet-I^SEC (Lox511I)^TEV::AID*::3xFLAG
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163093-plasmid-map-sequence-id-320623

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  19. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid
    #196881
    Purpose
    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    mOrange2-Cdk5rap2-CTD
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    196881-plasmid-map-sequence-id-391885

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  20. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    153551-plasmid-map-sequence-id-326384

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  21. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Expression
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

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  27. pAAV-Syn-FLEX-rc [ArchT-tdTomato]

    Plasmid
    #123607
    Purpose
    AAV mediated expression of ArchT-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. tdTomato has codons varied to reduce recombination. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Han et al Front Syst Neurosci. 2011;5:18. Epub 201
    Insert
    ArchT-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Syn
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123607-plasmid-map-sequence-id-243930

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  28. pJK275

    Plasmid
    #73363
    Purpose
    Encodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)
    Depositor
    T. J. Kappock
    Insert
    delta-8 sphingolipid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    missing residues 1-6
    Promoter
    GAL1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73363-plasmid-map-sequence-id-138870

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  29. pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

    Plasmid
    #110203
    Purpose
    Recipient vector for Cranial Neural Crest-specific expression of protein of interest
    Depositor
    Tatjana Sauka-Spengler
    Insert
    enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA
    Use
    Tags
    eGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110203-plasmid-map-sequence-id-210555

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  30. pCMV6-LacZ-BbsI

    Plasmid
    #114671
    Purpose
    For one-step cloning (with blue/ white screening) of an open reading frame of interest for constitutive gene expression
    Depositor
    Lynne Postovit
    Insert
    LacZ
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Lac promoter
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    114671-plasmid-map-sequence-id-224108

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  31. pJK369

    Plasmid
    #73594
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    FLAG tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73594-plasmid-map-sequence-id-136122

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  32. pJK137

    Plasmid
    #73313
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    5'-AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73313-plasmid-map-sequence-id-135010

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  33. pJK108

    Plasmid
    #73312
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73312-plasmid-map-sequence-id-135009

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  34. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

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  36. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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  39. pJK276

    Plasmid
    #73366
    Purpose
    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)
    Depositor
    T. J. Kappock
    Insert
    plastid omega-3 fatty acid desaturase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Mutation I231F. Also insert is missing residues 1…
    Promoter
    GAL1
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    73366-plasmid-map-sequence-id-135095

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  40. pJK142

    Plasmid
    #73317
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene. Encodes a glycine-204 to serine m…
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73317-plasmid-map-sequence-id-140432

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  44. pNGFP-BC

    Plasmid
    #161956
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161956-plasmid-map-sequence-id-319761

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  45. pCGFP-BC

    Plasmid
    #161959
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    GFPuv His8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161959-plasmid-map-sequence-id-319762

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  46. pNGFP-EU

    Plasmid
    #161967
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    EGFP His8
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161967-plasmid-map-sequence-id-319758

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  47. pCGFP-EU

    Plasmid
    #161968
    Purpose
    Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).
    Depositor
    Eric Gouaux
    Article
    Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1
    Type
    Empty backbone
    Use
    Tags
    EGFP His8
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    161968-plasmid-map-sequence-id-319729

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Showing: 3551 - 3600 of 18940 results