We narrowed to 4,048 results for: GAST
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Plasmid#146835PurposeInsect Expression of DmGW182-F961A-dsRNAresDepositorInsertDmGW182-F961A-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-PAM26B-dsRNAres_L
Plasmid#146837PurposeInsect Expression of DmGW182-PAM26B-dsRNAresDepositorInsertDmGW182-PAM26B-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW347_Ptbb2UTR-loxP-RFP-miniMOS
Plasmid#196064Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.DepositorInsertsmyo2p driven tagRFP
NeoR
Minimal Mos1
UseTagsExpressionWormMutationPromotermyo-2Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A, R280A,K284A]
Plasmid#188599PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG], R280A …PromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-DmBam_AG
Plasmid#148837PurposeMammalian Expression of DmBamDepositorInsertDmBam (bam Fly)
UseTagsExpressionMammalianMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-DmBam-L17EM24EL28EV32E_AG
Plasmid#148838PurposeMammalian Expression of DmBam-L17EM24EL28EV32EDepositorInsertDmBam-L17EM24EL28EV32E (bam Fly)
UseTagsExpressionMammalianMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-DmBam_AG
Plasmid#148840PurposeMammalian Expression of DmBamDepositorInsertDmBam (bam Fly)
UseTagsExpressionMammalianMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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piwi_sgRNA
Plasmid#186653Purposepiwi sgRNA plasmidDepositorInsertPiwi sgRNA Plasmid (piwi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P
Plasmid#147220PurposeBacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127DepositorInsertDmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA2
Plasmid#186664PurposeNbr C-tag sgRNA2 plasmidDepositorInsertNbr sgRNA 2 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA1
Plasmid#186663PurposeNbr C-tag sgRNA1 plasmidDepositorInsertNbr sgRNA 1 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
Plasmid#147209PurposeInsect Expression of DmXRN1-P1339AP1342A-dsRNAresDepositorInsertDmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
UseTagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvM-DmBam_141-442_AG
Plasmid#148833PurposeBacterial Expression of DmBam_141-422DepositorInsertDmBam_141-422 (bam Fly)
UseTagsExpressionBacterialMutationone non silent mutation A239 to S, described as …PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC
Plasmid#148402PurposeInsect Expression of DmNot1_1674-1962-dsRNAresDepositorInsertDmNot1_1674-1962-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMextli-M605D_AB
Plasmid#148344PurposeInsect Expression of DmMextli-M605DDepositorInsertDmMextli-M605D (mxt Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1711-2505-dsRNAres_R
Plasmid#147433PurposeInsect Expression of DmNot1_1711-2505-dsRNAresDepositorInsertDmNot1_1711-2505-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation4 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmMextli_577-620-I583RS590R-GB1_AB
Plasmid#148357PurposeBacterial Expression of DmMextli_577-620-I583RS590RDepositorInsertDmMextli_577-620-I583RS590R (mxt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-83-R56ER63E-GB1_AA
Plasmid#148272PurposeBacterial Expression of DmThor_50-83-R56ER63EDepositorInsertDmThor_50-83-R56ER63E (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1148_I
Plasmid#146604PurposeInsect Expression of DmNot1_1-1148DepositorInsertDmNot1_1-1148 (Not1 Fly)
UseTagsExpressionInsectMutation4 silent and two non silent N605S, K759M mutation…PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145995PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145985PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-LSm-del46-60-V5His6_C
Plasmid#146068PurposeInsect Expression of DmTral-LSm-del46-60DepositorInsertDmTral-LSm-del46-60 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C
Plasmid#146046PurposeInsect Expression of DmTral-LSm-del46-60DepositorInsertDmTral-LSm-del46-60 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCCR4_A
Plasmid#145894PurposeInsect Expression of DmCCR4DepositorInsertDmCCR4 (twin Fly)
UseTagsExpressionInsectMutationThe following variations comprises from our S2 ce…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG1856_A
Plasmid#145905PurposeInsect Expression of CG1856-3UTRDepositorInsertCG1856-3UTR (ttk Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-FLAG-NLS-EnR-MCS_N-term
Plasmid#136884PurposeDoxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)DepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-MCS-EnR_C-term
Plasmid#136885PurposeDoxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codonDepositorInsertengrailed repressor domain (en Fly)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorInsertssrp1 (Ssrp Fly)
UseTags6xHis and V5ExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-CTNNB1_S33Y
Plasmid#133376PurposePiggybac vector for constitutive expressionDepositorInsertCTNNB1 (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationS33YPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-Fc-His
Plasmid#72189PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-Fc-His
Plasmid#72187PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.23
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.26 EC10-AP-His
Plasmid#72063PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.26
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn1-3 (CG11739)
Plasmid#110642PurposeRetroviral expression of Flag-tagged Drosophila CG11739 in mammalian cellsDepositorInsertCG11739 (CG11739 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mei-S332 5.6Kb genomic DNA in Casper4
Plasmid#112984PurposeP element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genomeDepositorInsertmei-S332 (Sgo) (mei-S332 Fly)
UseP insertion vectorTagsGFPExpressionMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
UASp-deltaN cycB
Plasmid#112967PurposeP element vector for transposon insertion of nondegradable Cyclin B into Drosophila genomeDepositorInsertcycB (CycB Fly)
UseP insertion vectorTagsExpressionMutationN-term deletion of first 46 amino acids, includes…PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.30 EC10-AP-His
Plasmid#72064PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.30
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-AP-His
Plasmid#72060PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.22
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.23 EC10-AP-His
Plasmid#72061PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertDscam1_7.27.23
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dscam1_7.27.22 EC10-Fc-His
Plasmid#72186PurposeExpresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertDscam1_7.27.22
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF3H04
Plasmid#70971PurposeGateway entry cloneDepositorInsertTaf6 (Taf6 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only