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We narrowed to 4,786 results for: Mos

Showing: 3551 - 3600 of 4786 results

  1. Desmoplakin II tension sensor (FL-based)

    Plasmid
    #118721
    Purpose
    The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    inserted FL-based tension sensor module after aa1…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118721-plasmid-map-sequence-id-230467

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  7. Desmoplakin II no force control (FL-based)

    Plasmid
    #118724
    Purpose
    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118724-plasmid-map-sequence-id-230471

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  14. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229814-plasmid-map-sequence-id-460474

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  15. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid
    #229816
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-EGFP-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229816-plasmid-map-sequence-id-460476

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  17. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229841-plasmid-map-sequence-id-461093

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  20. pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

    Plasmid
    #224496
    Purpose
    Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    Actin-Vhh-sfGFP-P2A-HA-iRFP-caax
    Use
    Gateway cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    224496-plasmid-map-sequence-id-447065

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  26. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid
    #208966
    Purpose
    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208966-plasmid-map-sequence-id-422883

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  27. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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  30. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid
    #141100
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141100-plasmid-map-sequence-id-292288

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  40. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Use
    Tags
    Expression
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Promoter
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140942-plasmid-map-sequence-id-313600

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  41. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid
    #110324
    Purpose
    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Homo sapiens, Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110324-plasmid-map-sequence-id-213397

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  42. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid
    #110323
    Purpose
    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    HRASLS (PLAAT1 Homo sapiens, Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110323-plasmid-map-sequence-id-213396

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  44. Adeno BN

    Plasmid
    #101823
    Purpose
    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1
    Depositor
    Andrea Ventura
    Article
    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10
    Insert
    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    Use
    Adenoviral
    Tags
    FLAG-Cas9
    Expression
    Mutation
    Promoter
    Available since
    Nov. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  47. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid
    #217801
    Purpose
    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217801-plasmid-map-sequence-id-432878

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  49. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  50. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

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Showing: 3551 - 3600 of 4786 results