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We narrowed to 17,375 results for: puro

Showing: 3551 - 3600 of 17375 results

  1. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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  20. pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138145
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138145-plasmid-map-sequence-id-272531

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  28. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138146
    Purpose
    Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PspCas13b
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138146-plasmid-map-sequence-id-272533

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  38. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  41. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid
    #168106
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning
    Depositor
    Erich Jarvis
    Article
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Insert
    mCherry-KASH
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168106-plasmid-map-sequence-id-330309

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  44. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

    Plasmid
    #138144
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NES
    Expression
    Mutation
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138144-plasmid-map-sequence-id-272529

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Showing: 3551 - 3600 of 17375 results