Addgene

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CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP (GS)

Plasmid

#179637

Purpose

HCVP-inducible tri-transmembrane SEAP RELEASE with GS linkers

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-B2AD-hcvs-KKMP (GS)

Plasmid

#179639

Purpose

HCVP-inducible single TM SEAP RELEASE construct with GS linkers

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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GP940

Plasmid

#164539

Purpose

Expresses SmoPi-FLAG-Ub

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG-Ub

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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GP938

Plasmid

#164538

Purpose

Expresses SmoPi-FLAG

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened R455A (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R455A

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCL27-decoder

Plasmid

#159449

Purpose

Insert: Pfus1tet-citrine-tCyc1. The fluorescent protein Citrine is inducible by alpha-factor and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1tet-citrine-tCyc1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfus1tet

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD55

Plasmid

#158618

Purpose

Short Fibronectin FRET standard

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Short FRET Standard

Use

Tags

Expression

Bacterial

Mutation

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD56

Plasmid

#158619

Purpose

Long Fibronectin FRET Standard

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Long FRET Standard

Use

Tags

Expression

Bacterial

Mutation

Promoter

Xylose

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7-Rictor-shRNA

Plasmid

#157930

Purpose

Knockdown of Rictor

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Rictor shRNA

Use

Lentiviral and RNAi

Tags

tdTomato

Expression

Mutation

Promoter

mouse U6

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWS1859

Plasmid

#123065

Purpose

Positive regulator of Ste20

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste50 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS511

Plasmid

#123063

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS510

Plasmid

#123062

Purpose

Negative regulator of Ste12

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Dig1 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS509

Plasmid

#123061

Purpose

Negative regulator of Fus3

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Msg5 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS626

Plasmid

#123045

Purpose

P-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Mam2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS495

Plasmid

#123043

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pRNR2m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS497

Plasmid

#123042

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCUP1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS496

Plasmid

#123041

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pCYC1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS498

Plasmid

#123040

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pPHO5m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS486

Plasmid

#123039

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pGAL1m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS493

Plasmid

#123037

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pALD6m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS487

Plasmid

#123036

Purpose

Core promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTDH3m [2b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge