163,584 results

1. FLAG-tag

   Plasmid

   #55180

   Purpose

   FLAG-tag

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   FLAG
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClopHensorN

   Plasmid

   #50758

   Purpose

   Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

   Depositor

   Colin Akerman

   Article

   Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

   Insert

   ClopHensorN
   

   Use

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Promoter

   CAG

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-flag-GFP-iCAM-1

   Plasmid

   #205186

   Purpose

   This vector encodes of a synCAM (iCAM1) and GFP

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTK91_GFP-MRLC2

   Plasmid

   #46355

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

   Insert

   MRLC2 (MYL12B Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   pBABE

   Available Since

   July 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Integrin β5-GFP

   Plasmid

   #205090

   Purpose

   Express GFP-tagged human integrin β5 in mammalian cells

   Depositor

   Bianxiao Cui

   Article

   Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

   Insert

   Integrin beta 5 (ITGB5 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. IgK-IFN-luc

   Plasmid

   #14886

   Purpose

   Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

   Depositor

   David Baltimore

   Article

   Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

   Insert

   IgK-IFN (IFNB1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-fDIO-Cre

    Plasmid

    #121675

    Purpose

    Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

    Depositor

    Esteban Engel, Alexander Nectow

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    NLS-CRE-HA
    

    Use

    AAV

    Tags

    3xHA and SV40-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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