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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSN16

Plasmid

#100103

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to a 'strong' VP16 domain (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCART0016_CROPseq-CAR-Puro_GPC3-BBz

Plasmid

#242985

Purpose

GPC3-BBz CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

GPC3-BBz CAR

Use

Lentiviral

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0035_CROPseq-CAR-Puro_CD19-28z

Plasmid

#242983

Purpose

CD19-28z FMC63 CAR

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-28z FMC63 CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-hPGK-Blast-2A-STING-mNeonGreen

Plasmid

#227186

Purpose

Lentiviral expression plasmid encoding STING-mNeonGreen under hPGK promoter

Depositor

Nir Hacohen

Article

Gentili et al Cell Syst. 2024 Dec 18;15(12):1264-1277.

Insert

STING1 (STING1 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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SUPV3L1-HA G207V

Plasmid

#232349

Purpose

Expresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tag

Depositor

Samantha Lewis

Insert

SUPV3L1 (SUPV3L1 Human)

Tags

HA

Expression

Mammalian

Mutation

G207V

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-EFS-Blast-ARL13B

Plasmid

#227278

Purpose

Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-HsSAS6

Plasmid

#136790

Purpose

Mammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-SAS6 (SASS6 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pNR2F1.1.0-gDNA

Plasmid

#112484

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

Depositor

Kevin White

Insert

NR2F1 (NR2F1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTmycYBX1

Plasmid

#19878

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Y box binding protein 1 (YBX1 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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p27-mVenus reporter (PB)

Plasmid

#190731

Purpose

The p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

mVenus
p27

Expression

Bacterial and Mammalian

Mutation

p27-F62A-F64A

Promoter

EF1α

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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mEmerald-Nesprin3-C-18

Plasmid

#54203

Purpose

Localization: Nuclear Membrane , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Nesprin3 (SYNE3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

Plasmid

#223149

Purpose

Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TAP DHX36

Plasmid

#53547

Purpose

mammalian expression of DHX36

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

DHX36 (DHX36 Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 nonmutant

Plasmid

#183813

Purpose

Express CD74-ROS1 fusion (C6;R34) in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO SF-HERC2 (ShB-R)

Plasmid

#55613

Purpose

Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (WT) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Silent mutations have been incorporated into the …

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A Q67L

Plasmid

#28049

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

Q67L

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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