Addgene

We narrowed to 22,251 results for: Sele

Showing: 2191 - 2220 of 22251 results

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pQTEV-UFD1L

Plasmid

#31560

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

ubiquitin fusion degradation 1-like (UFD1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNA500

Plasmid

#149583

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Oct4-Cr1

Plasmid

#66989

Purpose

Expresses gRNA that targets stop codon for OCT4

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

gRNA for Crispr targeting the OCT4 stop codon

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk1b2

Plasmid

#13752

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1b2 (MAPK8 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pRVGP-BB

Plasmid

#15380

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-hST3GAL6-RFP

Plasmid

#22923

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human type 2 lactosamine alpha-2,3-sialyltransferase promoter driving DsRedExpress (ST3GAL6 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pQTEV-COPE

Plasmid

#31585

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

coatomer protein complex, subunit epsilon (COPE Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pHW539 (15xUAS::kin-2a(G310D)::SL2::GFP::let-858 3'UTR)

Plasmid

#107137

Purpose

Dominant negative PKA cGAL effector for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

kin-2a(G310D)::SL2::GFP

Use

Tags

Expression

Worm

Mutation

Promoter

15xUAS-delta-pes-10(basal promoter)

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2b2

Plasmid

#13757

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b2 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pQTEV-FBXO31

Plasmid

#34815

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

F-box protein 31 (FBXO31 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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SI16-Kit a

Plasmid

#58948

Purpose

expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Kit receptor a mouse monoclonal antibody (kita Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-POC5

Plasmid

#136786

Purpose

Mammalian expression of the centrosomal protein POC5 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-POC5 (POC5 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB225

Plasmid

#68616

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQsupR-Mi2b

Plasmid

#15379

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pLKO.1P shABCB7_2

Plasmid

#102969

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_2 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_1

Plasmid

#102968

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_1 (ABCB7 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Use

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
UAS:bloSwitch

Plasmid

#108355

Purpose

UAS driven green to red switch reporter for B3 recombinase expression

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]

Use

Zebrafish expression

Tags

Expression

Mutation

Zebrafish codon optimized

Promoter

lac

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSLiP-G1

Plasmid

#188963

Purpose

L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

dCas9
sgRNA: atcggtcgcattgttttccactagg

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PrhaBAD

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Pdha1

Plasmid

#175936

Purpose

knockout mouse Pdha1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Pdha1 gRNA (Pdha1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Ld

Plasmid

#135519

Purpose

Mammalian expression of myc-tagged H2-Ld

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Ld

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-6A-HA

Plasmid

#128103

Purpose

Express HA tagged Nxph4-6A

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-OPTN E50K

Plasmid

#175765

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

E50K

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 SKICH (1-140D)

Plasmid

#175768

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

SKICH (1-140D)

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQTEV-MAPRE1

Plasmid

#31589

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRRNA-G20-R20

Plasmid

#101822

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

Sven van Teeffelen, David Bikard

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Spy 1049

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hRREB1-RFP

Plasmid

#22924

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

human ras responsive element binding protein 1 driving DsRedExpress (RREB1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge