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pEE390

Plasmid

#149280

Purpose

T-DNA encoding TRV2 with gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX Smad3 C

Plasmid

#12634

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 C (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

C segment of Smad3 (aa 199-425)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pMEXC3GH (Apr)

Plasmid

#110083

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRJPaph-eBFP2

Plasmid

#67030

Purpose

Plasmid for Paph-eBFP2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Insert

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MKK4

Plasmid

#29579

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK4 (MAP2K4 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-shRalA-2

Plasmid

#15258

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pISI-mmP16 3' UTR

Plasmid

#22693

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mmP16 3'UTR

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
C-Ag15

Plasmid

#11868

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFAST NPC1(NTD)-3Q

Plasmid

#25772

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Tags

His

Expression

Insect

Mutation

aa23-252 only; N70Q N122Q N185Q

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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C-Ag20(0)

Plasmid

#11866

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
SI4-Jamc.2

Plasmid

#28216

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

Jamc.2 mouse monoclonal antibody (jam3a Zebrafish)

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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Kohinoor-H2B/pcDNA3

Plasmid

#67774

Purpose

Histon2B-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pECFP-Sec23A

Plasmid

#66610

Purpose

CFP fusion of Sec23A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec23A (SEC23A Human)

Tags

ECFP

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pILGFP3

Plasmid

#65451

Purpose

pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP reporter

Expression

Yeast

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pITGFP3

Plasmid

#65452

Purpose

pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Type

Empty backbone

Tags

yEGFP-CLN2PEST reporter

Expression

Yeast

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pWZLblast-RalA WT(RalA 402i-resistant)

Plasmid

#11124

Depositor

Christopher Counter

Article

Lim et al Cancer Cell. 2005 Jun . 7(6):533-45.

Insert

RalA WT (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

resistant to siRNA 402i

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pAGM55351

Plasmid

#169092

Purpose

MoClo level 1 35S promoter-BvADHα-Nos terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-BvADHα-Nos terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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RV-Cag-Dio-mEYFP

Plasmid

#87666

Purpose

retroviral vector encoding cre dependent expression of membrane EYFP

Depositor

Lynette Lim, Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Mem-EYFP

Use

Retroviral

Tags

palmitylation

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B

Plasmid

#21683

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

maternal expression at 31B (me31B Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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C-Ag20(8)

Plasmid

#11867

Depositor

Martin Smith

Article

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179077

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Neon(gfp)

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST51-LRRK2-R1441H

Plasmid

#25083

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arg 1441 mutated to His

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pEE387

Plasmid

#149285

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA1_FT:tNos

Expression

Plant

Promoter

35S

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2 enhancer- F1 LUC

Plasmid

#22426

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

eNOS promotor F1 (NOS3 Human)

Use

Luciferase

Tags

Luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 NL

Plasmid

#12632

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 NL (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-L segment of Smad3 (aa 1-211)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

Plasmid

#178996

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mOrange2-Paxillin-22

Plasmid

#57970

Purpose

Localization: Focal Adhesions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Paxillin

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAGM4723-Del

Plasmid

#112207

Purpose

Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

Depositor

Thomas Mock

Article

Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

Type

Empty backbone

Use

Golden gate assembly

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pfast NPC1(NTD)

Plasmid

#25773

Depositor

Johann Deisenhofer

Article

Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

Insert

npc1 (NPC1 Human)

Tags

His

Expression

Insect

Mutation

aa 23-252 only

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pJAZ10::LUC

Plasmid

#141172

Purpose

Luminescent reporter for jasmonic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtJAZ10 promoter

Use

Luciferase

Expression

Plant

Promoter

AtJAZ10, AT5G13220

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-FAK

Plasmid

#74478

Purpose

This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

Depositor

J Thomas Parsons

Article

Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

Insert

FAK (PTK2 Chicken)

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

Plasmid

#178995

Purpose

Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmTral

Plasmid

#21722

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAW-EGFP-4xlet-7 (pAW-AS27)

Plasmid

#40834

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Four target sites perfectly complementary to let-7

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-PGAL1-HBH

Plasmid

#26885

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag N-terminal

Use

Pcr tagging yeast

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pIK198

Plasmid

#65629

Purpose

"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ414-F53

Plasmid

#23077

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F30

Plasmid

#23066

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 9 to Glutamine H3 K9Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pJP72

Plasmid

#176479

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > GenR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJP102

Plasmid

#176480

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > NatR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJP103

Plasmid

#176481

Purpose

Expresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Inserts

actin > HygR
EF1 > mScarlet

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 17,800 results for: JUN