We narrowed to 3,025 results for: ER

1. meffBlue chromoprotein

   Plasmid

   #117845

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, meffBlue
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC-mCh-P2A-ST-EGFP-CAAX

   Plasmid

   #207636

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to mCherry and a SpyTag-EGFP localized to the cell membrane via CAAX tag

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-mCh-P2A-SpyTag-EGFP-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SpyCatcher’s critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV CAG-DO-TC66T-mCherry

   Plasmid

   #175178

   Purpose

   Cre-off TVA-mCherry fusion protein with 66T mutation

   Depositor

   Bernardo Sabatini

   Article

   Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

   Insert

   TVA
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   E66T

   Promoter

   CAG

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mDlx-SynTagMA_post-2A-mCerulean

   Plasmid

   #130899

   Purpose

   Synaptic Tag for Mapping Activity in GABAergic neurons

   Depositor

   Simon Wiegert

   Article

   Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

   Insert

   SynTagMA
   

   Use

   AAV

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEVOL-NBK-1

   Plasmid

   #207639

   Purpose

   A plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   Pyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The evolved synthetase NBK-1 contains mutations Y…

   Promoter

   araBAD

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX_TRC208-SYNJ2BP-V5

   Plasmid

   #92149

   Purpose

   Expresses V5-tagged human SYNJ2BP in mammalian cells.

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   SYNJ2BP-V5 (SYNJ2BP Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CMV

   Available since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-CaMPARI V398D

   Plasmid

   #60786

   Purpose

   Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

   Depositor

   Loren Looger, Eric Schreiter

   Article

   Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

   Insert

   CaMPARI V398D
   

   Use

   Tags

   Nuclear Export Signal

   Expression

   Mammalian

   Mutation

   V398D

   Promoter

   CMV

   Available since

   Nov. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSC-cUnaG-P2A-nUnaG-ST-P2A-mCh

   Plasmid

   #207634

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Peter Schultz, Jessica Seeliger

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-bHs

    Plasmid

    #134259

    Purpose

    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

    Depositor

    Edwin Manuel

    Article

    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

    Insert

    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    codon optimized for Salmonella typhimurium

    Promoter

    AraC

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-hspB7

    Plasmid

    #63098

    Purpose

    Expression of HSPB7 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb7 (HSPB7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ233 (dLbCpf1-SRDX)

    Plasmid

    #86211

    Purpose

    Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR

    Tags

    5' and 3' NLS and SRDX repressor

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. StrKDEL-IRES-ManII-mScarlet-i

    Plasmid

    #117274

    Purpose

    Expresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-i

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

    Inserts

    partial sequence of mannosidase II (Man2a1 Mouse)
    Streptavidin-KDEL
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV and IRES

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.Syn.GCaMP6m.WPRE.SV40

    Plasmid

    #100841

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6m
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …

    Promoter

    Synapsin

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. cjBlue chromoprotein

    Plasmid

    #117844

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses cjBlue chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, cjBlue
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p4A8_S7T_BC

    Plasmid

    #217832

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    VL: S7T

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Use

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-Mfn1-E209A

    Plasmid

    #141155

    Purpose

    Mammalian expression and labeling of mutant mitofusin 1 (E209A) deficient in GTP hydrolysis

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    MFN 1 (MFN1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    changed Glutamic acid at position 209 to Alanine …

    Promoter

    CMV

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. meffRed chromoprotein

    Plasmid

    #117836

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, meffRed
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge