We narrowed to 4,185 results for: Gca

1. lentiSAMv2 VEGFA-TSS-guide4

   Plasmid

   #125502

   Purpose

   CRISPR-mediated activation of VEGFA. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiSAMv2 C2CD4B-TSS-guide1

   Plasmid

   #125484

   Purpose

   CRISPR-mediated activation of C2CD4B. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR rs58692659-guide2

   Plasmid

   #125453

   Purpose

   CRISPR-mediated repression of human islet enhancer containing T2D-associated variant rs58692659 (ZFAND3 GWAS locus)

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

   Plasmid

   #118175

   Purpose

   CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CAMK1D TSS-guide2

   Plasmid

   #118178

   Purpose

   CRISPR-mediated repression of CAMK1D. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2

   Plasmid

   #118174

   Purpose

   CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

   Plasmid

   #177334

   Purpose

   bacterial expression of active SARS-CoV-2 3C-like proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pJMK019

   Plasmid

   #42168

   Depositor

   Adam Cohen

   Insert

   CaViar
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3 metaxin 1

   Plasmid

   #12418

   Depositor

   Paul Bornstein

   Insert

   metaxin (Mtx1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2006

   Availability

   Academic Institutions and Nonprofits only

10. LHZ1493

    Plasmid

    #222102

    Purpose

    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.

    Depositor

    Yongming Wang

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTJK459

    Plasmid

    #138008

    Purpose

    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Tami Kingsbury

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    U-A flip and hairpin extension: cgtttAagagctaTGCT…

    Promoter

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV Syn Barcode16

    Plasmid

    #226195

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode16
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV Syn Barcode15

    Plasmid

    #226189

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode15
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV Syn Barcode13

    Plasmid

    #226187

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode13
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOpen-PstIR

    Plasmid

    #165526

    Purpose

    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    PstI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. LHZ1494

    Plasmid

    #222104

    Purpose

    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

    Depositor

    Yongming Wang

    Insert

    sg1RNA, sg4RNA, sg7RNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV Syn Barcode17

    Plasmid

    #226190

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode17
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV Syn Barcode11

    Plasmid

    #226185

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode11
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTJK644

    Plasmid

    #138012

    Purpose

    SIX1 knockout

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA2 (SIX1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    U6

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTJK635

    Plasmid

    #138009

    Purpose

    SIX1 knockout

    Depositor

    Tami Kingsbury

    Insert

    SIX1 sgRNA2 (SIX1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge