We narrowed to 76,945 results for: rest

1. pX459-HypaCas9-mR2-ACTB_sgRNA

   Plasmid

   #183884

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. scAAV-TTR-mFgf15

   Plasmid

   #190594

   Purpose

   AAV construct containing Fgf15 cds under control of TTR promoter

   Depositor

   Roel Nusse

   Article

   Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

   Insert

   Fgf15 (Fgf15 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ttr

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB6 (human) FLAG pMSCV

   Plasmid

   #8534

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Mutation

   contains improved ribosomal binding site and FLAG…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pOPINK-ALG13 (OTU, aa 216-353)

   Plasmid

   #61414

   Purpose

   Expresses human ALG13 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-215 and aa 354-1137.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mPC1_4077-eGFP-3HA

   Plasmid

   #108390

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-mPC1_4119-3HA

   Plasmid

   #108395

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-KDEL_SBP-EGFP-CD59

   Plasmid

   #222307

   Purpose

   Synchronize the trafficking of CD59 from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)
   

   Expression

   Mammalian

   Mutation

   signal peptide removed

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mPC1_4119-eGFP-3HA

   Plasmid

   #108393

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mPC1_4077-3HA

   Plasmid

   #108392

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Tags

   3HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-mPC1_4077-eGFP

    Plasmid

    #108391

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid

    #172603

    Purpose

    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    The multiple cloning site (MCS) has been edited/e…

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-CaMKII-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153534

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1 KBTBD4 WT

    Plasmid

    #184627

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    no mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-Jaws-KGC-GFP-ER2

    Plasmid

    #65014

    Purpose

    AAV-mediated expression of Jaws under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV Retrograde, AAV5, and AAV8

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-Syn-Jaws-KGC-tdTomato-ER2

    Plasmid

    #153535

    Purpose

    AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-tdTomato-ER2
    

    Use

    AAV

    Tags

    ER2, KGC, and tdTomato

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    Syn

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Puro-Myr-Flag-IKBKE

    Plasmid

    #15293

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Myr-Flag

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pGL3-noSV40-humanEC1.45_min1-2

    Plasmid

    #173952

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45
    

    Use

    Luciferase

    Mutation

    None

    Promoter

    SV40 promoter

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBabe-Puro-Flag-IKBKE

    Plasmid

    #15292

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKBKE (IKBKE Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only