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Plasmid#213757PurposeBsaI-associated non-switchable methylaseDepositorInsertTruncated non-switchable methylase M2.BsaI
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1471
Plasmid#213759PurposeBsaI-associated switch methylaseDepositorInsertBsaI-associated switch methylase M.Osp807II
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1473
Plasmid#213761PurposeBpiI-associated switch methylaseDepositorInsertBpiI-associated switch methylase M2.NmeMC58II
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
POC1463
Plasmid#213141PurposeBsaI-associated non-switchable methylaseDepositorInsertNon-switchable methylase M2.Eco31I
UseTagsHis6-tagExpressionBacterialMutationPromoterT7Available sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC100
Plasmid#216369PurposexhNup155-Nb2i Nanobody productionDepositorInsertxhNup155-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC99
Plasmid#216370PurposexhNup155-Nb3i Nanobody productionDepositorInsertxhNup155-Nb3i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTP789
Plasmid#216376PurposexY-Nb1t (+2Cys) Nanobody productionDepositorInsertxY-Nb1t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC96
Plasmid#216354PurposexNup93-Nb1t Nanobody productionDepositorInsertxNup93-Nb1t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC115
Plasmid#216356PurposexNup93-Nb2t Nanobody productionDepositorInsertxNup93-Nb2t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC98
Plasmid#216358PurposexhNup93-Nb4i Nanobody productionDepositorInsertxhNup93-Nb4i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSC129
Plasmid#216362PurposexhNup98-Nb2i Nanobody productionDepositorInsertxhNup98-Nb2i
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDG04477
Plasmid#216352PurposexhNup35-Nb1t Nanobody productionDepositorInsertxhNup35-Nb1t
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPCTΩSP0021
Plasmid#187561PurposeModule for copper-inducible expression of firefly luciferase and nanoluc luciferase driven by minimal synthetic promoters with binding sites for CUP2DepositorInsertFirefly Luciferase
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa-C3
Plasmid#185587Purposeto recruit Vasa-C3 to membraneDepositorInsertVasa-C3
UseIn vitro transcription (mrna synthesis)TagsmCherry and membrane targeting sequence from Neur…ExpressionMutationC3 mutantPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa
Plasmid#185586Purposeto recruit Vasa to membraneDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsmCherry, S-Tag and membrane targeting sequence fr…ExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-His-SpyTag-XMod-Doc(WT)
Plasmid#157675PurposeExpression of XMod-Doc wild type with His6x tag and SpyTag.DepositorInsertXMod-Doc Wild type with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-Coh-His-SpyTag
Plasmid#157673PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.DepositorInsertCohesin (R.f) with Histag and SpyTag
UseTagsHistag and SpyTagExpressionBacterialMutationPromoterT5Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GeNL-Ca
Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsExpressionMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVEG16*
Plasmid#111855PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).DepositorInsertGAL3p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL3Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-2
Plasmid#175362PurposeG418R backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only