We narrowed to 5,867 results for: nls
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Plasmid#113023PurposeExpresses GCN4-dCas9 in mammalian cellsDepositorInsertGCN4-dCas9 (GCN4 S. pyogenes, Budding Yeast)
UseTagsExpressionMammalianMutationD10A, D839A, H840A, N863APromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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StrKDEL-IRES-mannosidase II-mTagBFP2
Plasmid#165460PurposeExpressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.DepositorInsertPartial ManII-mTag2BFP fusion (Man2a1 Mouse)
UseTagsStr-KDEL and mTag2BFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
hAID-BE3 (pRZ352)
Plasmid#131316PurposeCAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.DepositorInserthAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_LC
Plasmid#98661PurposeExpresses MBP-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST1374-dCas9-GCN4
Plasmid#113026PurposeExpresses dCas9-GCN4 in mammalian cellsDepositorInsertdCas9-GCN4 (GCN4 S. pyogenes, Budding Yeast)
UseTagsExpressionMammalianMutationD10A, H840A, N863APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1
Plasmid#101177PurposeHuman expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
UseTags3x FLAG and NLS (SV40)ExpressionMammalianMutationN497A/R661A/Q695A/K848A/Q926A/K1003A/R1060APromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_deltaLC
Plasmid#98664PurposeExpresses MBP-tagged hnRNPA2 without the LC domainDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationG190StopPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC1464
Plasmid#164881PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1DepositorInsertdcas13d-ADARdd
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_D290V
Plasmid#98663PurposeExpresses MBP-tagged full length hnRNPA2 with D290VDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationD290VPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG human Bmf
Plasmid#17242DepositorInsertBmf (BMF Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_P298L
Plasmid#104468Purposeexpress MBP-tagged full length hnRNPA2 P298LDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHis-MBPExpressionBacterialMutationP298LPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe 3xFLAG mouse Bmf
Plasmid#17243DepositorInsertBmf (Bmf Mouse)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-H840A
Plasmid#113024PurposeExpresses GCN4-H840A in mammalian cellsDepositorInsertGCN4-H840A (GCN4 S. pyogenes, Budding Yeast)
UseTagsExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable sinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST1374-H840A-GCN4
Plasmid#113027PurposeExpresses H840A-GCN4 in mammalian cellsDepositorInsertH840A-GCN4 (GCN4 S. pyogenes, Budding Yeast)
UseTagsExpressionMammalianMutationD839A, H840A, N863APromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
TET1CD-CRY2-EGFP
Plasmid#82555PurposeEncodes TET1 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe human Bmf
Plasmid#17240DepositorInsertBmf (BMF Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_P298L_S285C
Plasmid#104469Purposeexpress His tagged P298L S285C hnRNPA2 LCDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationP298L S285CPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY-GalVP16l (B694)
Plasmid#92030Purposeexpresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGalBD-VP16ExpressionMammalianMutationNLS sequence of CRY2 mutatedPromoterCMVAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBabe mouse Bmf
Plasmid#17241DepositorInsertBmf (Bmf Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE
Plasmid#228766Purposeinsert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27KDepositorInsertp27 kinase inhibitor (Cdkn1b Mouse)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
IscB-v3
Plasmid#227015PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-APOBEC-nCas9-Ung
Plasmid#198539PurposeExpresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-109
Plasmid#218200PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-054
Plasmid#218204PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-844
Plasmid#217998PurposeNucleoplasmin NLS for protein targeting to nucleusDepositorInsertNucleoplasmin NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2
Plasmid#218207PurposeZebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_1
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_6F→A
Plasmid#194269PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→ADepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationF276A, F283A, F289A, F367A, F397A, F401APromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_CR
Plasmid#194263PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CRDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM322A, M323A, M336A, M337A, M339A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_IDR
Plasmid#194264PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to AlanineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307A, M311A, M359A, M405A, M414A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
UseTags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→Y_IDR
Plasmid#194266PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR
Plasmid#194267PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sniper2P
Plasmid#193857PurposeExpresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-C7
Plasmid#194591PurposeBacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7 (S )
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only