We narrowed to 9,754 results for: Gnas

1. pLKO.1 shTGFBR3 puro

   Plasmid

   #58696

   Purpose

   Lentiviral shRNA vector for knockdown of human TGFBR3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   shTGFBR3 (TGFBR3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-Axin-IRES-GFP

   Plasmid

   #14716

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   Axin (Axin1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pJH2523

   Plasmid

   #191358

   Purpose

   Punc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFP

   Depositor

   Mei Zhen

   Article

   Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

   Insert

   GCaMP3
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-25

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOME0180

   Plasmid

   #178402

   Purpose

   Expresses KSHV ORF50 hyp tagged

   Depositor

   Dirk Dittmer

   Article

   Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

   Insert

   KSHV ORF 50
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV5 Flag-Smad2 EPSM

   Plasmid

   #14933

   Depositor

   Joan Massague

   Article

   Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

   Insert

   Smad2 EPSM (SMAD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Erk/Pro-directed kinase site mutant (EPSM). T220V…

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pBABE-Src-Rescue

   Plasmid

   #26983

   Depositor

   Joan Massague

   Article

   Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

   Insert

   c-Src (CSK Chicken)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. Tet-FRNK-GFP

   Plasmid

   #83472

   Purpose

   Lentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   FRNK (PTK2 Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   FRNK is the non-catalytic c-term domain of FAK ki…

   Promoter

   doxycycline-inducible promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pARF1-YFP

   Plasmid

   #11390

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 1 (ARF1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ROZA-XL

   Plasmid

   #64194

   Purpose

   Fluorescent reporter for ZAP-70 tyrosine kinase activity

   Depositor

   Annemarie Lellouch

   Article

   Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

   Insert

   ROZA-XL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ROZA-XL

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pCS2 mouse CV2 PC

    Plasmid

    #25776

    Depositor

    Edward De Robertis

    Article

    Coffinier et al Mech Dev. 2002 Dec;119 Suppl 1(Suppl 1):

    Insert

    cross veinless 2 (Bmper Mouse)
    

    Use

    Tags

    PC

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFlox_N-p300_Neo_FKBPF36V_mScarlet

    Plasmid

    #163886

    Purpose

    Acute Depletion of P300-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    P300 (Ep300 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 RL3Sc

    Plasmid

    #109365

    Purpose

    Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKD280.7

    Plasmid

    #173172

    Purpose

    Expresses SO_4387

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Insert

    SO_4387
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLIC-myc-GBAi

    Plasmid

    #171753

    Purpose

    For the mammalian expression of myc-GBAi

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Hoxb7 promoter

    Plasmid

    #21294

    Depositor

    Frank Costantini

    Article

    Srinivas et al Development. 1999 Apr . 126(7):1375-86.

    Insert

    Hoxb7 promoter (Hoxb7 Mouse)
    

    Use

    Promoter can be used to make transgenic mice

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

17. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Use

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Venus wGBD

    Plasmid

    #85222

    Purpose

    Venus tagged Cdc-42 sensor

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

    Insert

    wGBD (WASL Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Cdc42 binding domain of N-WASP

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac(His10-SNAP-Csk)

    Plasmid

    #177895

    Purpose

    Baculo expression of His10 tag fused to SNAPtag and human Csk

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Csk (CSK Human)
    

    Use

    Tags

    10xHis-TEV-TVMV-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEBG p130cas

    Plasmid

    #15001

    Depositor

    Raymond Birge

    Article

    Zvara et al Oncogene. 2001 Feb 22. 20(8):951-61.

    Insert

    p130cas (Bcar1 Rat)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pFlox c-CTCF FKBPF36V_EYFP_p2aBsd

    Plasmid

    #163870

    Purpose

    Acute Depletion of CTCF-EYFP

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Ctcf (Ctcf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. DEP-GFP

    Plasmid

    #109043

    Purpose

    Expression of the Dishevelled DEP domain

    Depositor

    Mariann Bienz

    Article

    Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

    Insert

    dishevelled segment polarity protein 2 (DVL2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Dvl2 DEP domain

    Promoter

    CMV

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid

    #118406

    Purpose

    Negative-control mutant for ExRai-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBABE puro mouse IRS-2

    Plasmid

    #11371

    Depositor

    Ronald Kahn

    Article

    Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

    Insert

    IRS-2 (Irs2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pCS2 Flag Smad3 EPSM V179T

    Plasmid

    #26998

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V S204A S208A S213A; V179T

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

26. pBABE bleo human IRS-1

    Plasmid

    #11448

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pSPAsp-hp

    Plasmid

    #48122

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    predicted optimal signal peptide for Bacillus megaterium protein secretion
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mVenus-RnBarr2

    Plasmid

    #137799

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFlox_N-RPB1_BSD_FKBPF36V_EYFP

    Plasmid

    #163884

    Purpose

    Acute Depletion of RBP1-EYFP

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Rbp1 (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pECFP-Rac1Y40C

    Plasmid

    #105293

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSIN-Ski-gRNA2

    Plasmid

    #180369

    Purpose

    targeting mouse Ski gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Ski targeting gRNA (Ski Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR-Arl13b2

    Plasmid

    #40871

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3-Lyn-sapphireAKAR

    Plasmid

    #118466

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-sapphireAKAR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LPCX Smad2 deltaSSMS

    Plasmid

    #12637

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Last 4 aa's deleted.

    Promoter

    Available since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Gi-c009

    Plasmid

    #173079

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    full length, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSW002-Pc-TorA(sp)

    Plasmid

    #111253

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA signal peptide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

    Plasmid

    #217708

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL3 (ADGRL3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-841

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSVE Grb2 myc 49L

    Plasmid

    #26085

    Depositor

    Bob Weinberg

    Article

    Egan et al Nature. 1993 May 6. 363(6424):45-51.

    Insert

    Grb2 P49L (GRB2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    P49L

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pDEST40-Arl13b89

    Plasmid

    #40874

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Tac-GFP

    Plasmid

    #162494

    Purpose

    Expresses Tac (IL2 recptor alpha subunit) with C-terminally tagged GFP in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    Tac (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Tmem98-sGFP2

    Plasmid

    #124503

    Purpose

    Expresses sGFP2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

    Depositor

    Renee van Amerongen

    Article

    van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

    Insert

    Tmem98 (Tmem98 Mouse)
    

    Use

    Tags

    sGFP2

    Expression

    Mammalian

    Mutation

    stop codon was removed to allow fusion with sGFP2…

    Promoter

    CMV

    Available since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. PhyB-mCherry-NLS

    Plasmid

    #51571

    Purpose

    Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    NLS and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available since

    Aug. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3.1(+)-ER-BSCaM2

    Plasmid

    #64855

    Purpose

    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-BSCaM2
    

    Use

    Tags

    CFP, N-terminal 27 amino acids from CYP450, and Y…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140230

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg (reverse orientation)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMH-SFB-RNF169

    Plasmid

    #99392

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

    Depositor

    Michael Huen

    Article

    Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. p6874 pHAGE-N-V5-MAML1-ADDAA

    Plasmid

    #37050

    Depositor

    Peter Howley

    Article

    Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

    Insert

    MAML1-ADDAA (MAML1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMVt

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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