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We narrowed to 6,480 results for: poly

Showing: 3751 - 3800 of 6480 results

  4. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  6. pGEM-myr-tdTomato-SV40pA-FKF

    Plasmid
    #119867
    Purpose
    Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    2x myristoylation
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119867-plasmid-map-sequence-id-239422

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  7. pGBW-m4046938

    Plasmid
    #145599
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145599-plasmid-map-sequence-id-300265

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  10. psicheck-2-Mut mod

    Plasmid
    #48165
    Purpose
    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element
    Depositor
    Vamsi Mootha
    Article
    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106
    Insert
    5" UTR of Mut (Mut Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    A/T mutation in the ATG of the uORF
    Promoter
    Available since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pGBW-m4046831

    Plasmid
    #145671
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145671-plasmid-map-sequence-id-319574

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  22. pKP4010

    Plasmid
    #113336
    Purpose
    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter
    Depositor
    Suzanne Sandmeyer
    Article
    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do
    Inserts
    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    Use
    Tags
    3X-FLAG/8X-Gly linker
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113336-plasmid-map-sequence-id-217436

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  34. pUBC-popTag-QtE-mScarlet

    Plasmid
    #240101
    Purpose
    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Encapsulin
    Use
    Lentiviral
    Tags
    mScarlet and popZ
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UBC promoter
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240101-plasmid-map-sequence-id-476613

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  35. pHR-PUM.HD-mCherry-Cry2WT

    Plasmid
    #240102
    Purpose
    Mammalian lentiviral vector for expressing the mCherry-tagged Pumilio homology domain fused to the light-inducible oligomerization domain CRY2.
    Depositor
    Liam Holt
    Article
    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e
    Insert
    Pumilio homology domain
    Use
    Lentiviral
    Tags
    mCherry and Cry2
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    SFFV promoter
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    240102-plasmid-map-sequence-id-476612

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  36. pYihI-N-term D-N/E-Q

    Plasmid
    #233095
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to asparagine (…
    Promoter
    Available since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233095-plasmid-map-sequence-id-468389

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  37. pYihI-N-term D-A/E-L

    Plasmid
    #233097
    Purpose
    Expression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Depositor
    Michael Downey
    Article
    Baijal et al bioRxiv 2025.02.12.637445
    Insert
    YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    N-term aspartic acids (D) mutated to alanine (A) …
    Promoter
    Available since
    May 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233097-plasmid-map-sequence-id-468390

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  38. pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)

    Plasmid
    #233880
    Purpose
    Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (–)-Eldh1 (Elen_0511 Eggerthella lenta)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233880-plasmid-map-sequence-id-467497

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  39. pXD80EN8-Pct3-(+)-Eddh(cHis6)-noAccs(ElA2)

    Plasmid
    #233879
    Purpose
    Expresses His-tagged Eggerthella lenta A2 (+)-Eddh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eddh (Elen_0501 Eggerthella lenta)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233879-plasmid-map-sequence-id-467495

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  40. pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

    Plasmid
    #233874
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233874-plasmid-map-sequence-id-467490

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  41. pXD80ENSF14-Pct3-ElVadh(cHis6)-Accs(ElDSM2243)

    Plasmid
    #233871
    Purpose
    Expresses His-tagged Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    El Vadh (Elen_0497 Eggerthella lenta)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233871-plasmid-map-sequence-id-467488

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  42. pXD80ENSF12-Pct3-(–)-Eldh1(cHis6)-Accs(ElA2)

    Plasmid
    #233870
    Purpose
    Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (–)-Eldh1 (Elen_0511 Eggerthella lenta)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233870-plasmid-map-sequence-id-467487

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  46. pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

    Plasmid
    #233875
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233875-plasmid-map-sequence-id-463816

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Showing: 3751 - 3800 of 6480 results