We narrowed to 120,127 results for: CIL

1. pJT84_GalL_nCDA1Δ194-BE3

   Plasmid

   #145047

   Purpose

   Expresses nCDA1Δ194-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ194-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-194AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT78_GalL_nCDA1Δ195-BE3

   Plasmid

   #145044

   Purpose

   Expresses nCDA1Δ195-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ195-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   pmCDA1(1-195AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET14b-dabAB2 D353A

   Plasmid

   #133016

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET14b-dabAB2 C351A

   Plasmid

   #133014

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Mutation

   C351A

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9FnCpf1GG

   Plasmid

   #131013

   Purpose

   Backbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.

   Depositor

   Chase Beisel

   Article

   Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

   Inserts

   direct repeat of SpCas9
   GFP expression cassette
   direct repeat of FnCas12a
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVR2

   Plasmid

   #84719

   Purpose

   E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   12 nt stretch lacking A's inserted before 5&…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. LP451

   Plasmid

   #90183

   Purpose

   pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 841-1426

   Depositor

   Lotte Pedersen

   Article

   Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

   Insert

   NPHP4 (NPHP4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   aa residues 841-1426

   Promoter

   CMV

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 mCherry Gamma 3

   Plasmid

   #65234

   Purpose

   To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

   Depositor

   Narasimhan Gautam

   Article

   Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

   Inserts

   mcherry
   Gng3 (Gng3 Mouse)
   

   Tags

   6-His tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pPB-CAG-Mbd3b-pA-pgk-bsd

   Plasmid

   #74920

   Purpose

   Mammalian expression of Mbd3b

   Depositor

   Jose Silva

   Article

   dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

   Insert

   Mnd3b (Mbd3 Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pPB-CAG-Mbd3c-pA-pgk-bsd

    Plasmid

    #74921

    Purpose

    Mammalian expression of Mbd3c

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Insert

    Mbd3c (Mbd3 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pVRa31_34

    Plasmid

    #49687

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF31_34
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVRa30_35

    Plasmid

    #49685

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF30_35
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag

    Plasmid

    #41013

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1189-1192 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

    Plasmid

    #41012

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1185-1188 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

    Plasmid

    #41011

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid

    #138147

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-Rab11-WT

    Plasmid

    #101047

    Purpose

    Expresses HA-tagged human Rab11-WT in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    RAB11 (RAB11A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFl Strep-sumo-TEV-hAgo2

    Plasmid

    #136235

    Purpose

    Expresses human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Strep Sumo

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. p23-mNeongreen-DDX21 WT-mRuby3

    Plasmid

    #179225

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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