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Plasmid#235310PurposeComMAND closed-loop circuit regulating mRuby2, EFS-drivenDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEFS (human EF1a short, intronless)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH
Plasmid#235261PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF4x1-mRuby2-miRE.FF4-bGH
Plasmid#235291PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF4 design 3DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF5x1-mRuby2(miRE.FF5)-bGH
Plasmid#235263PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 2DepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF6x2-bGH
Plasmid#235292PurposeComMAND open-loop circuit regulating mRuby2 with 2 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1a (human EF1a)Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
hSyn-HA-PGRN-LAMP1TM-IRES-GFP
Plasmid#234877PurposeLentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.DepositorInsertProgranulin (GRN Human)
UseLentiviralTagsHA tag and LAMP-1 transmembrane domain and cytoso…ExpressionMammalianMutationPromoterhSYnAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)
Plasmid#232411PurposeAAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HRAS(G12V)-IRES-p53DD
Plasmid#233186PurposeGateway entry vector for HRAS(G12V) and p53DD (RIDD)DepositorUseOtherTagsExpressionMutationG12VPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-p53DD-IRES-HRAS(G12V)
Plasmid#233187PurposeRetroviral expression of p53DD and HRAS(G12V) (DDIR)DepositorUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-p53DD-IRES-HRAS(G12V)
Plasmid#233188PurposeGateway entry vector for p53DD and HRAS(G12V) (DDIR)DepositorUseOtherTagsExpressionMutationPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Const.4 EGFP-IRES-Luc-PS9
Plasmid#231908PurposeFor packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)DepositorInsertEGFP-IRES-Luc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-SS-VN155(I152L)-IRE1-dLD
Plasmid#217752PurposeExpresses the VN fragment of BiFC-IRE1-dLDDepositorInsertSerine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
UseTagsVenus (1-154, I152L)ExpressionMammalianMutationdeleted amino acids 29-408PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MYC-GFP-IRES-mCherry
Plasmid#231232PurposeMYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2-MCRS1-GFP-IRES-mCherry
Plasmid#231231PurposeMCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.DepositorInsertMCRS1 (MCRS1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNG-IRES-mScarlet-dMCP
Plasmid#231089PurposeBicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.DepositorInsertmNG-IRES-mScarlet-dMCP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-2xESP3I-IRES-mCherry
Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV-FoxP3-R337Q-IRES-Thy1.1
Plasmid#229617PurposeMammalian expression of full length mouse FoxP3 (R337Q)DepositorInsertfull length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
UseTagsExpressionMammalianMutationR337QPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV-FoxP3-A372S-IRES-Thy1.1
Plasmid#229618PurposeMammalian expression of full length mouse FoxP3 (A372S)DepositorInsertfull length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
UseTagsExpressionMammalianMutationA372SPromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDY2259 hNOLC1 atgRNA Paired Guide 1
Plasmid#220989PurposehNOLC1 atgRNADepositorInsertNOLC1 atgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY2260 hNOLC1 atgRNA Paired Guide 2
Plasmid#220990PurposehNOLC1 atgRNADepositorInsertNOLC1 atgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only