We narrowed to 2,695 results for: SR

1. pHAGE-N'-FLAG-HA-NAP1S318A

   Plasmid

   #195410

   Purpose

   Expresses FLAG-HA tagged human NAP1 with S318A point mutation

   Depositor

   Alicia Pickrell

   Article

   Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

   Insert

   Nak associated protein 1 (AZI2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Serine 318 turned to Alanine

   Promoter

   Available since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. W118-1_hZBTB7B_R363L

   Plasmid

   #180381

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R363L (ZBTB7B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R363L

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. W118-1_hZBTB7B_D35N_R49Q

   Plasmid

   #180383

   Purpose

   lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_D35N/R49Q (ZBTB7B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D35N/R49Q

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. W118-1_hZBTB7B_R49H

   Plasmid

   #180382

   Purpose

   lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   ZBTB7B_R49H (ZBTB7B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R49H

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV.CBA.YFP.miR-E_shRps6ka2-3

   Plasmid

   #180390

   Purpose

   Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   shRps6ka2-3 (Rps6ka2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV.CBA.YFP.miR-E_shRps6ka2-2

   Plasmid

   #180389

   Purpose

   Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   shRps6ka2-2 (Rps6ka2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV.CBA.YFP.miR-E_shRps6ka2-1

   Plasmid

   #180388

   Purpose

   Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

   Depositor

   Huda Zoghbi

   Article

   Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

   Insert

   shRps6ka2-1 (Rps6ka2 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

   Plasmid

   #171100

   Purpose

   CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9 (Neurog2 Mouse, S. pyogenes)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Ascl1 x2)

    Plasmid

    #171099

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Ascl1 Mouse, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAPTrap-Clover-Muro

    Plasmid

    #82334

    Purpose

    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    Clover-T2AMuro
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGAPTrap-eGFP-IRESMeo

    Plasmid

    #82502

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP-IRESMeo
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST26 Ago2 4A

    Plasmid

    #59907

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    mutated four serines to alanines

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBZSA FPR1 ECFP

    Plasmid

    #66725

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Heini Miettinen

    Insert

    FPR1 (haplotype 3)-ECFP (FPR1 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    SR-alpha

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOP-HTT-HR65Q

    Plasmid

    #92249

    Purpose

    HTT donor DNA with 65Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJOP-HTT-HR45Q

    Plasmid

    #92248

    Purpose

    HTT donor DNA with 45Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CaMKII-ChrimsonR-tdTomato

    Plasmid

    #99231

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CaMKII promoter. tdTomato has codons varied between the first and second tandem repeats to reduce recombination. Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CaMKII

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid

    #122064

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1a（1.1kb short version)

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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