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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Publication

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GAL4BD-VP16/pDON207

Plasmid

#201230

Purpose

GAL4-VP16 transcription factor

Depositor

Michael Ayliffe

Publication

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

GAL4BD-VP16

Expression

Plant

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pflareG-psd95-exon18

Plasmid

#90266

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Publication

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Insert

Psd95-exon18 (Dlg4 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT/TO/HIS/FLAG/HA-YTHDF2

Plasmid

#38089

Depositor

Markus Landthaler

Publication

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

YTHDF2 YTH domain family, member 2 (YTHDF2 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEF-DEST51-GOLPH3

Plasmid

#21690

Depositor

Lynda Chin

Publication

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Invitrogen gateway destination vector

Tags

6xHIS and V5

Expression

Mammalian

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFRT-DHX36iso1

Plasmid

#159585

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

Depositor

Markus Hafner

Publication

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p221z-CAS9p-TagRFP-t35s

Plasmid

#118386

Purpose

Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Publication

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p-TagRFP

Use

CRISPR

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMMB67EH-Spy

Plasmid

#90102

Purpose

IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

Depositor

Samuel Miller

Publication

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YcgR (ycgR )

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pShuttle-HA-FLAG-TIN2

Plasmid

#35025

Depositor

Sheila Stewart

Publication

Nittis et al Mol Cell Proteomics. 2010 Jun;9(6):1144-

Insert

TIN2 (TINF2 Human)

Use

Adenoviral

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

Plasmid

#223712

Purpose

Expresses autophagy flux LC3B fluorescence reporter

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

LC3B (MAP1LC3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

mCherry::EGFP::LC3B

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-SNAP-KanMX6

Plasmid

#87024

Purpose

Tag S. pombe genes in C terminal with SNAP

Depositor

Julien Berro

Publication

Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

Insert

SNAP

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toshinobu Nakamura, Toru Nakano

Publication

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Publication

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Publication

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Tags

GST

Expression

Bacterial

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.5

Plasmid

#155032

Purpose

Fluorescent reporter for ATF4 translation

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Publication

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTOX1

Plasmid

#127446

Purpose

Encodes YhaV toxin. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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