We narrowed to 13,583 results for: TIM

1. pLNCX2-lifeact-mAPPLE

   Plasmid

   #89820

   Purpose

   mAPPLE labeled fluorescent actin

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   lifeact-mAPPLE
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yoEmerald-Kan

   Plasmid

   #44946

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoEmerald
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVHed02

   Plasmid

   #82352

   Purpose

   Bxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmid

   Depositor

   Richard Murray

   Article

   Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

   Inserts

   mKate2 RFP
   Superfolder GFP
   Temporal logic gate with TP901 and Bxb1 attachment sites
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRSET FLIPglu-2uDelta13

   Plasmid

   #13561

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIPglu F16F D152A Delta13
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   F16A, D152A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. csr-1_son-1_mScarlet

   Plasmid

   #191745

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Use

   Tags

   NCU04502 promoter and mScarlet

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YeNL-H2B_pcDNA3

   Plasmid

   #89522

   Purpose

   Yellow color variant of bright luminescent protein, fused to histone 2B

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   YeNL
   

   Use

   Tags

   histone 2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-yoPS-CFP2-Kan

   Plasmid

   #44948

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-yoPS-CFP2-SpHis5

   Plasmid

   #44853

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPS-CFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pVD_85

   Plasmid

   #149604

   Purpose

   (parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

   Depositor

   Michael Jensen

   Article

   Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

   Insert

   XylR
   

   Use

   Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-oChEF-citrine

    Plasmid

    #50975

    Purpose

    AAV transfer vector with mammalian codon-optimized ChEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-dH3-YFP

    Plasmid

    #8694

    Depositor

    Kami Ahmad

    Article

    Janicki et al Cell 2004 Mar 5;116(5):683-98.

    Insert

    H3 (His3:CG31613 Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CyclinB-GFP

    Plasmid

    #54465

    Purpose

    CyclinB-GFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

    Insert

    CyclinB
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K67E and I150T mutations compared to GenBank refe…

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYES2.1-pomMet17

    Plasmid

    #107453

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met17 (met17 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOS031: superecliptic pHluorin (endoplasmic reticulum)

    Plasmid

    #163051

    Purpose

    superecliptic pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-yoLSS-mKate2-SpHis5

    Plasmid

    #44848

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoLSS-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. csr-1_son-1_mApple

    Plasmid

    #191741

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and mApple

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. csr-1_son-1_mCherry

    Plasmid

    #191742

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Use

    Tags

    NCU04502 promoter and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tubulin-CeNL_pcDNA3

    Plasmid

    #89515

    Purpose

    Cyan color variant of bright luminescent protein, fused to b-tubulin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Use

    Tags

    b-tubulin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPRv2-7SK-Blast

    Plasmid

    #183620

    Purpose

    lentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.

    Depositor

    Bill Sugden

    Article

    Hutcheson et al ACS Synth Biol. 2022 Jun 29. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Modified 7SK

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHT-trxA.ads

    Plasmid

    #47383

    Purpose

    Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Insert

    ads
    

    Use

    Tags

    6xHis and trxA

    Expression

    Bacterial

    Mutation

    Promoter

    Pgrac

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSUPER-retro-puro-shNup133-1

    Plasmid

    #87334

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCIS.MTRIA-PRLH

    Plasmid

    #184618

    Purpose

    For expression of a fluorescent sensor for Prolactin-releasing peptide factor in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-PRLH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCIS.MTRIA-NOC

    Plasmid

    #184611

    Purpose

    For expression of a fluorescent sensor for nociceptin in mammalian cells

    Depositor

    Hiroshi Hibino, Daisuke Ino

    Article

    Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

    Insert

    MTRIA-NOC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YeNL-Actin_pcDNA3

    Plasmid

    #89521

    Purpose

    Yellow color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    YeNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNB777

    Plasmid

    #60153

    Purpose

    Red click beetle luciferase (Promega) driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR-L4-pTF-L1R

    Plasmid

    #45954

    Depositor

    Patrick Salmon

    Article

    Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

    Insert

    pTF promoter
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    TET optimized

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pME-QF2w-SV40pA

    Plasmid

    #155117

    Purpose

    Middle entry vector containing QF2w

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QF2w
    

    Use

    Gateway middle entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge