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Showing: 1521 - 1540 of 1768 results

  1. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  2. pIND4-RC-C2-MmXYRS

    Plasmid
    #222744
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222744-plasmid-map-sequence-id-444741

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  3. pScarlessHD-DsRed-w+

    Plasmid
    #80801
    Purpose
    Vector for generating dsDNA donors for HDR. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    piggyBac-3xP3-DsRed
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80801-plasmid-map-sequence-id-152801

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  5. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Natalie Krahn, Dieter Söll
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  6. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid
    #231805
    Purpose
    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.
    Depositor
    Annette Oxenius
    Insert
    eGFP-mCEP83
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231805-plasmid-map-sequence-id-460689

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  7. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Tags
    Expression
    Mutation
    Promoter
    3xP3 and GMR::hsp70
    Available since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

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  8. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

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  9. XLone-BSD NFIL3 T2A Puro

    Plasmid
    #140028
    Purpose
    Tunable and temporal expression control of NFIL3 and puro resistant gene
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    puromycin
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140028-plasmid-map-sequence-id-275842

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  12. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  13. DN2-Gli3 in pECE

    Plasmid
    #121971
    Purpose
    medium Gli3 transcriptional activator
    Depositor
    James Briscoe
    Insert
    DN2Gli3 (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus deletion (nt1-1401)
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121971-plasmid-map-sequence-id-241250

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  15. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  20. EcpH-TM

    Plasmid
    #85389
    Purpose
    Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses
    Depositor
    Gregory Melikyan
    Article
    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do
    Insert
    ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85389-plasmid-map-sequence-id-169209

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Showing: 1521 - 1540 of 1768 results