We narrowed to 2,369 results for: puc19
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Plasmid#239750Purposeguide for aeBlueDepositorInsertguide targeting aeBlue
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
ExpressionYeastPromoterPGK1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcHELIX-sgRNA_entry (CJT94)
Plasmid#181783PurposeExpresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFS-ccdB-12X MS2_mPGK-Puro
Plasmid#177809PurposeAssembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'DepositorTypeEmpty backboneTags12X MS2 stem loopsExpressionMammalianPromoterEFSAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ3538 eforRed-targeting sgRNA
Plasmid#239455Purposeguide for eforRedDepositorInsertguide targeting eforRed
UseCRISPR; Cell-free system using bacterial extractExpressionBacterialPromoterJ23119Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCE38
Plasmid#174432PurposeC. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE41
Plasmid#174433PurposeC. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.DepositorInsertHYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
UseCRISPRExpressionBacterial and YeastAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry
Plasmid#177182Purposeplasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertpegRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-gRNA
Plasmid#61089PurposeCRISPR/Cas9 system gRNA cloningDepositorInsertH1 promoter; gRNA sequences
UseCRISPR; /cas9 grna cloning vectorExpressionMammalianPromoterH1Available SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry
Plasmid#177183Purposeplasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry markerDepositorInsertsgRNA targeting the PEAR-GFP plasmid
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LMNA_mScarlet-I_Donor
Plasmid#178092PurposeHDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.DepositorInsertLMNA-mScarlet-I HDR donor
UseCRISPRExpressionMammalianAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDAS12342_PEAR-GFP_2in1
Plasmid#177184PurposePEAR-GFP plasmid with a pegRNA that targets its own plasmidDepositorInsertsEGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid
UseCRISPRExpressionMammalianMutationdisrupted 5' splice sitePromoterCMV and U6Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL0-BvDODAα1
Plasmid#162528PurposeBeta vulgaris 4,5-DOPA dioxygenase alpha 1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.DepositorInsertDODAα1
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9748-dCBE
Plasmid#163008Purposeplasmid expressing CBE with dCas9DepositorInsertdCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianPromoterEF1-aAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only