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pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP

Plasmid

#117141

Purpose

Express gRNA vs Dmap1

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

gDmap1 v2 (Dmap1 Synthetic)

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Promoter

human U6 promoter, mouse PGK promoter

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

Plasmid

#107578

Purpose

B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

GFP+

Use

Synthetic Biology

Tags

B. megaterium DSM319 xylA leader sequence (MTSSKI…

Expression

Bacterial

Mutation

F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

Promoter

fbp promoter Bacillus megaterium DSM319

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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HIPK2 gRNA (BRDN0001148687)

Plasmid

#76749

Purpose

3rd generation lentiviral gRNA plasmid targeting human HIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HIPK2 (HIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HIPK2 gRNA (BRDN0001162198)

Plasmid

#76751

Purpose

3rd generation lentiviral gRNA plasmid targeting human HIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HIPK2 (HIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HIPK2 gRNA (BRDN0001147868)

Plasmid

#76752

Purpose

3rd generation lentiviral gRNA plasmid targeting human HIPK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HIPK2 (HIPK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DHFR.dn-cHSF1

Plasmid

#134731

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pPD0055_pIVTRup_MS2-p65-HSF1

Plasmid

#242541

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

MCP-p65-HSF1 (RELA, HSF1 Human)

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV_ACE2-Flag_Hygro

Plasmid

#191998

Purpose

Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-CFP

Plasmid

#55794

Purpose

Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_ETS1_WT

Plasmid

#82118

Purpose

Gateway Donor vector containing ETS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ETS1 (ETS1 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KIAA1549_BRAF

Plasmid

#205838

Purpose

Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA1549_BRAF (KIAA1549, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pGMC00030 (aka pRC0707)

Plasmid

#209673

Purpose

Lentiviral vector expressing PP7-P65-HSF1

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

PP7-P65-HSF1 (HSF1, RELA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-puro.WPRE (MT126)

Plasmid

#145839

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.4(732)-IgG1

Plasmid

#154106

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase enzyme

Plasmid

#67515

Purpose

Recruitable human type IV 5-phosphatase enzyme

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ACE2-his expression plasmid

Plasmid

#158089

Purpose

Expresses ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A13

Plasmid

#132038

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A13 (SLC25A13 Human)

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_BRAF_p.G469V

Plasmid

#82833

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G469V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-EGR3-P2A-mCherry

Plasmid

#203861

Purpose

Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

EGR3 (EGR3 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PMXS-GOT1

Plasmid

#72872

Purpose

The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

GOT1 (GOT1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

sgRNA resistant human GOT1, 7 silent mutations c1…

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE-BRAF-N581S

Plasmid

#116188

Purpose

Lentiviral expression of BRAF N581S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

N581S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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