2,226 results

1. pEF Doc2alpha D291N mutant GFP

   Plasmid

   #128821

   Purpose

   To visualize Doc2alpha D291N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D291N

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF Doc2beta D303N mutant GFP

   Plasmid

   #128822

   Purpose

   To visualize Doc2beta D303N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D303N

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF Doc2beta 6X mutant GFP

   Plasmid

   #128823

   Purpose

   To visualize Doc2beta 6X mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D163A, D218A, D220N, D303A, D357A, D359A

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

   Plasmid

   #113156

   Purpose

   An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat Rosa26
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2-HKII-D

   Plasmid

   #64516

   Purpose

   AS-30D hepatoma hexokinase II 1.0 kbp promoter-luciferase reporter vector

   Depositor

   Peter Pedersen

   Article

   Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

   Insert

   Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
   

   Use

   Luciferase

   Tags

   firefly luciferase

   Expression

   Mutation

   Isolated from AS-30D rat hepatoma

   Promoter

   Hexokinase II promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pRK5-TGF beta type I receptor (mL45TD)

   Plasmid

   #34702

   Depositor

   Ying Zhang

   Article

   Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T202D, N265A, D267A, N268A

   Promoter

   CMV

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

7. mTFP1-Cx26-7

   Plasmid

   #55473

   Purpose

   Localization: Gap Junctions, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Cx26
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCK032

   Plasmid

   #105412

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   Hormone-binding domain (glucocorticoid receptor)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI/ BpiI restriction sites removed

   Promoter

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM rat islet1 (phoII) [TJ#1]

   Plasmid

   #16268

   Depositor

   Thomas Jessell

   Insert

   islet1 (Isl1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

10. mCit-Snap25b D179K A199R

    Plasmid

    #53259

    Purpose

    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    SNAP-25B (Snap25 Rat)
    

    Use

    Tags

    4aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    D179K and A199R

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

11. mMaroon-Cx43-7

    Plasmid

    #56198

    Purpose

    Localization: Gap Junctions, Excitation: 610, Emission: 652

    Depositor

    Michael Davidson

    Insert

    Cx43
    

    Use

    Tags

    mMaroon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Myc-delta/gamma2SIL

    Plasmid

    #119731

    Purpose

    GABAA receptor expression (chimeric rat delta subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRD/GABRG2 (Gabrg2, Gabrd Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. G protein alpha-o-R179C-EE

    Plasmid

    #55780

    Purpose

    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    

    Use

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Bacterial and Mammalian

    Mutation

    Arginine 179 was replaced with cysteine

    Promoter

    CMV

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-Fos F343A

    Plasmid

    #8969

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F343A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pSG5-FLAG-CaMKKbeta rat K193A

    Plasmid

    #32450

    Depositor

    Anthony Means

    Article

    Anderson et al Cell Metab. 2008 May;7(5):377-88.

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K193A (S3V, G96R, and P108L also present)

    Promoter

    SV40

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pVenus-PICK1-shRNA

    Plasmid

    #72575

    Purpose

    Expresses shRNA against PICK1 as well as the marker Venus fluorescent protein

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

    Insert

    PICK1 (Pick1, Pick1 Mouse, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-PTEN-deltaPDZ

    Plasmid

    #110182

    Purpose

    Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

    Depositor

    Jose Esteban

    Article

    Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

    Insert

    PTEN (Pten Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of last 4 amino acids

    Promoter

    CMV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PS-mOrange-Homer1-N-18

    Plasmid

    #57810

    Purpose

    Localization: Integrin/Focal Adhesions, Excitation: 548 / 634, Emission: 565 / 662

    Depositor

    Michael Davidson

    Insert

    Homer1 (Homer1 Rat)
    

    Use

    Tags

    PS-mOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. XE129 beta2AR Fz1-pcDNA3.1

    Plasmid

    #16795

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz1 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Chimera of beta 2 adrenergic receptor (extracellu…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pSuper-PlexinB1

    Plasmid

    #115174

    Purpose

    shRNA against rodent PlexinB1

    Depositor

    Suzanne Paradis

    Article

    McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

    Insert

    shRNA targeting Plxnb1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge