Addgene

We narrowed to 12,997 results for: SHI

Showing: 3901 - 3950 of 12997 results

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.EF1a.FLEX.MTRIA-OT

Plasmid

#184595

Purpose

For production of AAV for a fluorescent sensor for oxytocin

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

AAV

Tags

Expression

Mutation

Promoter

Human elongation factor-1 alpha promoter

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-mutOdd

Plasmid

#18044

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

mutant Odd Skipped

Use

Tags

Expression

Bacterial

Mutation

A mutant version of the zinc finger protein Odd S…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-5HT

Plasmid

#184597

Purpose

For expression of a fluorescent sensor for serotonin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-5HT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NRCoC

Plasmid

#74300

Purpose

Mammalian expression of full-length treacle fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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726-Desmoglein2-myc

Plasmid

#32232

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmoglein2 (DSG2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

human beta-actin

Available since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-NPY

Plasmid

#184613

Purpose

For expression of a fluorescent sensor for neuropeptide Y in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-NPY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T1-Smad7-HA

Plasmid

#13506

Depositor

Jeff Wrana

Article

Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

Insert

SMAD7 (SMAD7 Human)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pCIS.MTRIA-SST

Plasmid

#184619

Purpose

For expression of a fluorescent sensor for somatostatin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-SST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMGA-plux-sfGFP

Plasmid

#139936

Purpose

AHL-inducible sfGFP expression on M. magneticum/E. coli shuttle vector

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

LuxR-Plux-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 4, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Cyto-GCaMP3

Plasmid

#44331

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

GCaMP3

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p043 mTcf-4 B

Plasmid

#11031

Depositor

Ramesh Shivdasani

Article

Lee et al J Biol Chem. 1999 Jan 15. 274(3):1566-72

Insert

Tcf-4 (Tcf7l2 Mouse)

Use

Xenopus, avian, and zebrafish expression

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Expression

Mutation

Promoter

Available since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
pCIS.MTRIA-ENK

Plasmid

#184604

Purpose

For expression of a fluorescent sensor for enkephalin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-ENK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS-MEYFP

Plasmid

#86372

Purpose

membrane targeted EYFP expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

EYFP

Use

Zebrafish

Tags

GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1 gfaABC1D-Lck-GCaMP3

Plasmid

#44330

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Insert

Lck-GCaMP3

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-FRBx2

Plasmid

#193571

Purpose

Expresses mCherry tagged with two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mCherry

Use

Tags

FRB (T2098L)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB (TIREVOL)-mEos3.2

Plasmid

#189720

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZS-YP_009701829

Plasmid

#198377

Purpose

Express YP_009701829 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_009701829 (CVA-1_407L )

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB (TIRSTD)-sfGFP

Plasmid

#189674

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-AGT

Plasmid

#184598

Purpose

For expression of a fluorescent sensor for angiotensin II in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-AGT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC-SEAP #M004#725

Plasmid

#42125

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

alkaline phosphatase, placental (SEAP) (ALPP Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDEST17-Nanog

Plasmid

#13988

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
1.6 kB VEGF-luc

Plasmid

#29667

Depositor

Patricia D'Amore

Article

Shima et al J Biol Chem. 1996 Feb 16. 271(7):3877-83

Insert

1.6 kB VEGF

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-Chimeric_Butterfly_YR_1.0

Plasmid

#59801

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Yellow-Red Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
KFS2MT

Plasmid

#44624

Depositor

Robert Oshima

Article

Cecena et al Am J Pathol. 2006 Jan;168(1):310-20.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

polyomavirus middle T antigen (PyMT)

Expression

Mutation

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB (TIRSTD)-mNG

Plasmid

#189721

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV500 A-SREBP2

Plasmid

#33358

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Insert

SREBP2

Use

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only
pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Use

Tags

4XFLAG

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA10(gfcD)

Plasmid

#176191

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA10(gfcD-10xHis)

Plasmid

#176192

Purpose

gfcD protein expression

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSA10(gfcBCD)

Plasmid

#176193

Purpose

expresses 3 overlapping genes (gfcB, gfcC, gfcD) from this one operon

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcBCD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGK415-rtetTAK8N, L131L

Plasmid

#165969

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Mutation

Promoter

pPGK1

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-CCK

Plasmid

#184602

Purpose

For expression of a fluorescent sensor for CCK in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-CCK

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB (TIREVOL)-PAmCherry1

Plasmid

#189718

Purpose

titratable expression of PAmCherry1 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

PAmCherry1

Use

Tags

His-T7-EK

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDC.015

Plasmid

#184992

Purpose

Express -Eco2 RT and ncRNA in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco2: RT and ncRNA(wt), a1/a2 length: 13

Use

Tags

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rap1b E63

Plasmid

#118328

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b E63 (RAP1B Bovine)

Use

Tags

GFP

Expression

Mammalian

Mutation

Q63E

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19 PKC zeta

Plasmid

#8425

Depositor

Frederic Mushinski

Article

Goodnight et al Gene 1992 Dec 15;122(2):305-11.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Use

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Promoter

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pUAS-MmTFP13xmyc

Plasmid

#86371

Purpose

membrane targeted Tfp1 expression in zebrafish

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

mTfp1

Use

Zebrafish

Tags

3xMyc and GAP43 membrane targeting

Expression

Mutation

Promoter

UAS

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Pyb1301(JBEI-11629)

Plasmid

#87959

Purpose

jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro selection

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6Rosa-CAG-Cas9

Plasmid

#118998

Purpose

Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PTEN-H123Y

Plasmid

#28299

Depositor

Maria-Magdalena Georgescu

Article

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Insert

PTEN (PTEN Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Phosphatase Inactive Mutant His123Tyr

Promoter

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
CΔ1

Plasmid

#74296

Purpose

Mammalian expression of treacle (residues 1176-1302) fused to GFP

Depositor

Rita Shiang

Article

Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.

Insert

treacle ribosome biogenesis factor 1 (Tcof1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Expresses residues 1176 - 1302

Promoter

CMV

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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