We narrowed to 11,805 results for: NSI;
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pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGB-S reporter
Plasmid#207841PurposeA three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coliDepositorInsertsRpoH sensing construct
SOS sensing construct
RpoS sensing construct
ExpressionBacterialPromoterPosmY, PsulA, and grpEAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF4-2xCSTREP
Plasmid#136169PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF4DepositorInsertORF4
ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF39-2xCSTREP
Plasmid#136200PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39DepositorInsertORF39
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-ABK
Plasmid#126035PurposeEfficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.DepositorInsertstRNA synthetase 1
tRNA synthetase 2
tRNA for TAG codon
ExpressionBacterialMutationL274M, C313A, Y349FPromoteraraBAD promoter, glnS promoter, and proK promoterAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNTI647 dCas9-Mxi1 TetR KanMX
Plasmid#139474PurposeIntegrates CRISPRi effector and tet-responsive regulatorDepositorInsertsdCas9-Mxi1
Tet Repressor
UseCRISPRTagsMxi1 and NLSExpressionYeastMutationD10A, H840APromoterpGPM1 and pTEF1Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP(LaG2) PAGER(TF)
Plasmid#229996PurposeExpresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF37-2xCSTREP
Plasmid#136198PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37DepositorInsertORF37
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4 CoV-2 nsp1 3xFLAG
Plasmid#176057PurposeExpressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.DepositorAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLuc
Plasmid#83281Purposerecombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)DepositorInsertFirefly Luciferase
UseAAVExpressionMammalianPromoterCAGAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-10xCTD
Plasmid#138472PurposeExpresses GST-tagged 10xCTD heptapeptide repeat in bacteriaDepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-4xCTD
Plasmid#138471PurposeExpresses GST-tagged 4xCTD heptapeptide repeat in bacteriaDepositorAvailable SinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Blast Twist ER
Plasmid#18799DepositorInserttwist (Twist1 Mouse)
UseRetroviralTagsER hormone binding domainExpressionMammalianMutationG525R mutation in the ER. The presence of the G52…Available SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF8-2xCSTREP
Plasmid#136172PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8DepositorInsertORF8
ExpressionMammalianPromoterCMVAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-K8.1-2xCSTREP
Plasmid#136157PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8.1DepositorInsertK8.1
ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-eGFP-attP(bxb)-*BsdR
Plasmid#183751PurposeBxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox251
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox
Plasmid#183763PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorInsertccdB
UseCre/Lox and Synthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
VRK1-dTag-puro
Plasmid#199651PurposeExpresses VRK1 C-term. Tagged with dTAGDepositorAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-K5-2xCSTREP
Plasmid#136154PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5DepositorInsertK5
ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only
