Skip to main content
Addgene

We narrowed to 7,007 results for: tet

Showing: 1561 - 1580 of 7007 results
  1. pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT

    Plasmid
    #139176
    Purpose
    SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone.
    Depositor
    Insert
    CaMXMT
    Use
    Transposon
    Tags
    dTomato-P2A
    Expression
    Mammalian
    Mutation
    P131T on dTomato (Please see depositor comments)
    Promoter
    tight TRE-controlled CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Expression
    Mutation
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid
    #202569
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112669
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1561 - 1580 of 7007 results