159,238 results

1. pLV-Puro-{CMV-CuO}>{MCS}:T2A:mCherry

   Plasmid

   #236249

   Purpose

   Puromycin selected lentiviral vector with MCS for cumate-inducible gene, for use with dual-inducible CymR-CuO system

   Depositor

   Genevieve Kendall

   Article

   Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-CuO

   Available since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTUM4

   Plasmid

   #230905

   Purpose

   Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

   Depositor

   Arne Skerra

   Article

   Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

   Inserts

   FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
   SurA (surA E. coli)
   DsbA (dsbA E. coli)
   DsbC (dsbC E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-mitoGFP

   Plasmid

   #44385

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoGFP (COX8A Human)
   

   Use

   Lentiviral

   Tags

   Cox8 targeting sequence and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV2/1

   Plasmid

   #112862

   Purpose

   AAV packaging plasmid expressing Rep/Cap genes

   Depositor

   James M. Wilson

   Insert

   Rep2/Cap1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Truncated P5

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. psPAX2-D64V

   Plasmid

   #63586

   Purpose

   psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

   Depositor

   Andrew Scharenberg, David Rawlings

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Packaging plasmid for integrase deficient lentiviral vectors
   

   Use

   Integrase deficient lentiviral packaging vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PEmax

   Plasmid

   #174820

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. eeBxb1_IVT

   Plasmid

   #222342

   Purpose

   Plasmid for evolved and engineered Bxb1 mRNA in vitro transcription template

   Depositor

   David Liu

   Article

   Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

   Insert

   Codon-optimized evolved and engineered Bxb1
   

   Use

   Template for in vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive (derepressed P-lac)

   Available since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAG/MNase

    Plasmid

    #123461

    Purpose

    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

    Depositor

    Steven Henikoff

    Article

    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

    Insert

    Protein A-Protein G-Micrococcal Nuclease
    

    Use

    Tags

    6xHIS and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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