We narrowed to 383 results for: Pef1

1. attP-puro-GA donor

   Plasmid

   #182140

   Purpose

   attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_PDCD6_EF-hand

   Plasmid

   #110009

   Purpose

   Protein expression and purification of PDCD6_EF-hand

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PDCD6_EF-hand (PDCD6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AYBEv0.1

   Plasmid

   #193966

   Purpose

   A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(wt)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a, CBh

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FH-TET3-HxDmut-pEF

   Plasmid

   #127896

   Purpose

   Expresses ten-eleven-translocation 3 (TET3) containing HxD mutation at H1077Y and D1079A

   Depositor

   Patrick Hogan, Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   TET3-HxDmut (TET3 Human)
   

   Use

   Tags

   HA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FH-TET3-CXXCmut-pEF

   Plasmid

   #127895

   Purpose

   Expresses ten-eleven-translocation 3 (TET3) containing CXXC mutation 80THQ82 to AAA

   Depositor

   Patrick Hogan, Anjana Rao

   Article

   Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

   Insert

   TET3-CxxCmut (TET3 Human)
   

   Use

   Tags

   HA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMpGWB118

   Plasmid

   #68572

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMpGWB218

   Plasmid

   #68609

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGWB424

   Plasmid

   #68689

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Triple repeats of Citrine (3xCitrine)

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AYBEv3

   Plasmid

   #193967

   Purpose

   An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(v3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G163R, N169S, S198A, K202A, G203A, S206A, K210A

   Promoter

   hU6, EF1a, CBh

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)

    Plasmid

    #211767

    Purpose

    dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    dCas9, Spy, Snoop, aGCN4, AviTags
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    dCas9 D10A and H840A

    Promoter

    pGK and pEF1a

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Use

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMpGWB321

    Plasmid

    #68649

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMpGWB121

    Plasmid

    #68575

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMpGWB221

    Plasmid

    #68612

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMpGWB418

    Plasmid

    #68683

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMpGWB421

    Plasmid

    #68686

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FH-TET1-pEF

    Plasmid

    #49792

    Purpose

    This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

    Depositor

    Anjana Rao

    Article

    Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

    Insert

    Tet methylcytosine dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB337

    Plasmid

    #68061

    Purpose

    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter

    Depositor

    Takayuki Kohchi

    Article

    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Full human ELL2

    Plasmid

    #127253

    Purpose

    Expresses full-length human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Myc-Idax WT

    Plasmid

    #68553

    Purpose

    This plasmid expresses full length IDAX.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    IDAX (Cxxc4 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. L2_2xNOP1gRNA-Cas9-CsA

    Plasmid

    #136140

    Purpose

    Plasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 563aa ELL2 pEF4B

    Plasmid

    #127273

    Purpose

    Expresses 563aa human WT ELL2

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 truncated at SphI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains 564 N-terminal amino acids ELL2, termina…

    Promoter

    pEF1a

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HA tagged mid ELL2

    Plasmid

    #127274

    Purpose

    Expresses human ELL2 with HA tag in the middle that does not disrupt function

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 with HA at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA inserted at XbaI after Met 186

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. COOHend ELL2

    Plasmid

    #127255

    Purpose

    Expresses the C terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (C-terminal end) (ELL2 Human)
    

    Use

    Other

    Tags

    Expression

    Mammalian

    Mutation

    contains only the last 389 aa

    Promoter

    pEF1a

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NH2end ELL2

    Plasmid

    #127252

    Purpose

    Expresses the N terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (1-287) (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    contains only amino acids 1-289

    Promoter

    pEF1a

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1a-TET1-dCas9

    Plasmid

    #235593

    Purpose

    Tet1CD fused to N-terminus of dCas9

    Depositor

    Ting Zhou

    Article

    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

    Insert

    TET1-dCas9 (TET1 Human, SpCas9 is from Streptococcus pyogenes)
    

    Use

    CRISPR

    Tags

    3xNLS, TET1 CD, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJT305 Pa01 Lenti pEF-attB LSR GFP

    Plasmid

    #184948

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FH-Tet2-pEF

    Plasmid

    #41710

    Purpose

    This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

    Insert

    tet methylcytosine dioxygenase 2 (Tet2 Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJT306 Kp03 Lenti pEF-attB LSR GFP

    Plasmid

    #184949

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Kp03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJT302 Ec03 Lenti pEF-attB LSR GFP

    Plasmid

    #184945

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec03 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJT303 Ec04 Lenti pEF-attB LSR GFP

    Plasmid

    #184946

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec04 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pJT304 Ec07 Lenti pEF-attB LSR GFP

    Plasmid

    #184947

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Lacra Bintu, Ami Bhatt, Patrick Hsu, Michael Bassik

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Ec07 Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Myc-Idax DBM

    Plasmid

    #68554

    Purpose

    This plasmid expresses full length IDAX containing a DNA binding mutation.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    IDAX-DBM (Cxxc4 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T162A, H164A and Q165A mutations

    Promoter

    EF1a

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 289aa Met133, 138, 186Ileu ELL2

    Plasmid

    #127272

    Purpose

    Expresses 289aa human ELL2 with Met133, 138, 186Ileu

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met133, 138, 186 Ileu, cut with XbaI blunt fillin

    Promoter

    pEF1a

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. gCBEv2

    Plasmid

    #220617

    Purpose

    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    hUNG2-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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