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We narrowed to 446 results for: DHFR

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Showing: 351 - 400 of 446 results

  1. MUT_S7-H1

    Plasmid
    #218396
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218396-plasmid-map-sequence-id-433914

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  3. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  4. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218395-plasmid-map-sequence-id-433911

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  12. pDownstream1Lox

    Plasmid
    #164574
    Purpose
    Integration of 1 LoxN site in the genome of P. berghei
    Depositor
    Olivier Silvie
    Type
    Empty backbone
    Use
    Cre/Lox
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164574-plasmid-map-sequence-id-325425

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  21. pAAH2

    Plasmid
    #41826
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41826-plasmid-map-sequence-id-69077

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  22. pAAH3

    Plasmid
    #41827
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41827-plasmid-map-sequence-id-69168

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  25. pAAH4

    Plasmid
    #41828
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41828-plasmid-map-sequence-id-69199

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  26. pGPΩTp

    Plasmid
    #130660
    Purpose
    Insertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.
    Depositor
    Miguel Valvano
    Article
    Flannagan et al Infect Immun. 2007 Apr;75(4):1679-89. Ep
    Insert
    Ω trimethoprim cassette
    Expression
    Bacterial
    Mutation
    Addition of Ω sites next to trimethoprim cassette…
    Promoter
    dhfr promoter
    Available Since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    130660-plasmid-map-sequence-id-258172

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  27. pGJJ045

    Plasmid
    #183621
    Purpose
    abundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183621-plasmid-map-sequence-id-361478

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  28. pGJJ001

    Plasmid
    #183619
    Purpose
    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.
    Depositor
    Ben Lehner
    Article
    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Available Since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183619-plasmid-map-sequence-id-361476

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  30. pUpstream2Lox

    Plasmid
    #164573
    Purpose
    Integration of 2 LoxN sites in the genome of P. berghei
    Depositor
    Olivier Silvie
    Inserts
    Green fluorescent protein - human dihydrofolate reductase (GFP-2A-hDHFR) (DHFR Human, Synthetic)
    bifunctional yeast enzyme cytosine deaminase and uridyl phosphoribosyl transferase (yFCU) (FCY1, FUR1 Budding Yeast)
    Use
    Cre/Lox
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164573-plasmid-map-sequence-id-325424

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  31. pAAH1

    Plasmid
    #41825
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available Since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41825-plasmid-map-sequence-id-56469

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  33. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Tags
    3xFLAG
    Expression
    Yeast
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  45. 2xFKBP-mCh-KDEL

    Plasmid
    #173010
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-mCh-KDEL
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173010-plasmid-map-sequence-id-339203

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  46. 2xFKBP-smFP(myc)-KDEL

    Plasmid
    #173021
    Purpose
    FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of spaghetti monster FP (myc, non-fluorescent). IRES following ORF.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    2xFKBP-smFP(myc)-KDEL
    Tags
    FKBP and smFP(myc) non-fluorescent
    Expression
    Mammalian
    Available Since
    Aug. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    173021-plasmid-map-sequence-id-339210

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Showing: 351 - 400 of 446 results