Addgene

We narrowed to 18,313 results for: Tec

Showing: 351 - 400 of 18313 results

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BB2_L_12_syn_A_B

Plasmid

#90268

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSB

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_12_syn_A_C

Plasmid

#90269

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_12_syn_A_D

Plasmid

#90270

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSC

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_12_syn_A_E

Plasmid

#90271

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSE

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_12_syn_A_H

Plasmid

#90274

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSH

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_12_syn_A_I

Plasmid

#90275

Purpose

BB2 Linker containing between FS1 and FS2 the BB3 linker with FSA and FSI

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMST637_BB3_L_AE_hyg_KanOri_pyrG5

Plasmid

#90282

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AE

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMST639_BB3_L_AG_hyg_KanOri_pyrG5

Plasmid

#90284

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AG

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMST635- BB3_L_AC_hyg_KanOri_pyrG5

Plasmid

#90280

Purpose

BB3 recipient for homologous integration in pyrG locus with linker AC

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT104

Plasmid

#25088

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

Edge detector delta luxI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pNGFP-BC

Plasmid

#161956

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

GFPuv His8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGFP-BC

Plasmid

#161959

Purpose

Used for rapid and efficient membrane protein precrystallization screening strategy utilizing green fluorescent protein and fluorescence-detection size-exclusion chromatography (FSEC).

Depositor

Eric Gouaux

Article

Kawate et al Structure. 2006 Apr;14(4):673-81. doi: 1

Type

Empty backbone

Use

Tags

GFPuv His8

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
Ub-G76V-GFP

Plasmid

#11941

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-G76V-GFP

Use

Tags

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Promoter

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pZE-PalkB-GFP

Plasmid

#44381

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Inserts

GFP
PalkB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PalkB

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pACYC-PLlambda-alkSm4-3

Plasmid

#44383

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkSm4-3

Use

Synthetic Biology

Tags

Expression

Mutation

Q410I, S470T, K546E, E775D

Promoter

PLlambda

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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P4-TadCBEd

Plasmid

#225143

Purpose

Packaging plasmid for TadCBEd in v3b PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-TadCBEd

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ub-M-GFP

Plasmid

#11938

Depositor

Nico Dantuma

Article

Dantuma et al Nat Biotechnol. 2000 May . 18(5):538-43.

Insert

Ub-M-GFP

Use

Tags

Expression

Mammalian

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
V309 - pDNR MCS SA lacZ alpha - Donor

Plasmid

#11692

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Cin85-3SH3-pCMV6-AN-GFP

Plasmid

#51137

Purpose

Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells

Depositor

Agnieszka Swiatecka-Urban

Insert

Cin85 (SH3KBP1 Human)

Use

Tags

TurboGFP

Expression

Mammalian

Mutation

contains 3 SH3 domains

Promoter

CMV

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only
TRMPVIR

Plasmid

#27994

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPVIR (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pLibacceptorV2

Plasmid

#106250

Purpose

Recombination-mediated cassette exchange with target genomic loci

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-PLlambda-alkS(WT)

Plasmid

#44382

Depositor

Hal Alper

Article

Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

Insert

AlkS(WT)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLlambda

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
V308 pDNR MCS lacZ alpha - Donor

Plasmid

#11691

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
TRMPV-Hygro

Plasmid

#27996

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-Hygro (shLuci-1309)

Use

RNAi and Retroviral; Fluorescent reporters

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
TRMPV-Neo

Plasmid

#27990

Depositor

Scott Lowe

Article

Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.

Insert

TRMPV-Neo (shRen)

Use

RNAi and Retroviral; Fluorescent reporters

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_P_12_asn_mbfA

Plasmid

#90289

Purpose

BB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mbfA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
LBJJ432

Plasmid

#117508

Purpose

AtEC_enh. promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pEC_enh:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

EC1.2:EC1.1

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_P_12_asn_coxA

Plasmid

#90290

Purpose

BB1 promoter coxA gene of Aspergillus niger between FS1 and FS2

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

coxA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_L_23_syn_BsaI

Plasmid

#89915

Purpose

Linker to construct BB1 from PCR product with FS2 and FS3 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
V677 pDNR MCS BE lacZ alpha - Donor

Plasmid

#11694

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
V954 - pDNR MCS SA no SA CmR - Donor

Plasmid

#11697

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMpGWB303-MpIGPDm

Plasmid

#188555

Purpose

Binary vector for MpIGPDm and mALS as selective markers in Marchantia polymorpha (igpd mutants)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

MpIGPDm and mALS

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_EF_syn_BbsI

Plasmid

#89921

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSE and FSF (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_HI_syn_BbsI

Plasmid

#89924

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSH and FSI (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_T_34_ani_trpC

Plasmid

#90291

Purpose

BB1 terminator trpC gene Aspergillus nidulans between FS3 and FS4

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

trpC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_L_12_syn_BsaI

Plasmid

#89914

Purpose

Linker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB1_L_34_syn_BsaI

Plasmid

#89916

Purpose

Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_AB_syn_BbsI

Plasmid

#89917

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSA and FSB (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_BC_syn_BbsI

Plasmid

#89918

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSB and FSC (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_CD_syn_BbsI

Plasmid

#89919

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSC and FSD (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_DE_syn_BbsI

Plasmid

#89920

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSD and FSE (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_FG_syn_BbsI

Plasmid

#89922

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSF and FSG (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
BB2_L_GH_syn_BbsI

Plasmid

#89923

Purpose

Linker to construct BB2 from BB1(FS1 - FS4) with FSG and FSH (in with BbsI, out with BsaI)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TEVSH

Plasmid

#125194

Purpose

His-tagged TEV protease with solubility increasing mutations

Depositor

Helena Berglund

Article

van den Berg et al J Biotechnol. 2006 Feb 10;121(3):291-8.

Insert

TEV_SH

Use

Tags

V5 His-tag

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Lifeact-mCherry

Plasmid

#193300

Purpose

mammalian transient expression - expression of Lifeact-mCherry

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lifeact-Halo

Plasmid

#193328

Purpose

mammalian transient expression - expression of Lifeact-Halo

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

Plasmid

#231128

Purpose

Expresses xylanase via a cSAT2.0 scheme

Depositor

Xiaofeng Yang

Article

Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

Insert

xylanase

Use

Tags

cSAT2.0

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBlueKan+cysMPro

Plasmid

#187879

Purpose

Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

CysM

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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