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pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

Plasmid

#86420

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

Plasmid

#220075

Purpose

expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL5 H478/9A (OSBPL5 Human)

Expression

Mammalian

Mutation

H478/9A, deltaTMD

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX-(3×FLAG)-TEV-ProtA

Plasmid

#52693

Purpose

For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

Depositor

Michael Nick Boddy

Insert

(3×FLAG)-TEV-ProtA

Expression

Yeast

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL3-deltaTM

Plasmid

#109025

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-deltaTM (ATL3 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Hygro/pTER shATM #2 (w639-1)

Plasmid

#22532

Depositor

Eric Campeau

Insert

sh Ataxia telangiectasia mutated

Use

Lentiviral and RNAi

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNATM3.1/V5-His TOPO-PKCi-K274W

Plasmid

#55690

Purpose

expresses dominant negative human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E and K274W

Promoter

CMV

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
pcDNATM3.1/V5-His TOPO-PKCi-A120E

Plasmid

#55687

Purpose

expresses constitutively active human PKCi tagged with V5

Depositor

Pedro Salas

Article

Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

Insert

Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)

Tags

V5/His

Expression

Mammalian

Mutation

A120E

Promoter

CMV

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-natMX-tetO-Pcyc1-3xFLAG

Plasmid

#41021

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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Arabidopsis thaliana Atm3 d80 pET21a+

Plasmid

#173045

Purpose

Expresses AtAtm3 in E.coli

Depositor

Douglas Rees

Article

Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

Insert

Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)

Tags

6xHis tag

Expression

Bacterial

Mutation

with N-terminal 80 amino acids deletion

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL2-deltaTM

Plasmid

#109021

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-deltaTM (ATL2 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Zeo/pTER shATM #2 (735-1)

Plasmid

#22528

Depositor

Eric Campeau

Insert

sh Ataxia telangiectasia mutated

Use

Lentiviral and RNAi

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-FLAG-APEX2-NES-NatMX6

Plasmid

#229231

Purpose

Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

Depositor

Fulvio Reggiori

Article

Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmATM

Plasmid

#208392

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgATM (Atm Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

Plasmid

#179719

Purpose

Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

Plasmid

#179723

Purpose

Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)

Plasmid

#86421

Purpose

N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequence

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

CDC42pr-KAR2SS-GFP1-10

Tags

GFP1-10

Expression

Yeast

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Expression

Yeast

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

Plasmid

#149277

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-BRCA1-mCherry

Plasmid

#195275

Purpose

express mCherry-BRCA1 fusion

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

mCherry-BRCA1

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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