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We narrowed to 746 results for: streptococcus -(crispr) -(cas9)

Showing: 141 - 160 of 746 results

  2. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

    Plasmid
    #101211
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101211-plasmid-map-sequence-id-198053

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  3. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

    Plasmid
    #101204
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101204-plasmid-map-sequence-id-198050

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  4. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid
    #101210
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101210-plasmid-map-sequence-id-198133

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  5. pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant

    Plasmid
    #101203
    Purpose
    Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/M1–N497,GGS,V713–D1368
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, M1-N497, GGS, V713-D13…
    Promoter
    T7
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    101203-plasmid-map-sequence-id-197572

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  6. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid
    #101212
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101212-plasmid-map-sequence-id-197040

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  8. pHCas9-Nours

    Plasmid
    #107733
    Purpose
    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Human optimized
    Promoter
    TEF1
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107733-plasmid-map-sequence-id-208542

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  12. pHis-parallel1-NLSH2BCas9

    Plasmid
    #112065
    Purpose
    Expression and purification of NLSH2BCas9 for fungal gene editing
    Depositor
    Jeff Coleman
    Article
    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10
    Insert
    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    Use
    CRISPR
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Cas9 was codon optimized for Aspergillus niger
    Promoter
    promoter in vector
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112065-plasmid-map-sequence-id-217190

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  20. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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Showing: 141 - 160 of 746 results