We narrowed to 22,825 results for: Sis

1. pDGE1109

   Plasmid

   #202773

   Purpose

   CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:TREX2-zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK

   Plasmid

   #62670

   Purpose

   Vector for PhiC31o mRNA synthesis

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM126

   Plasmid

   #105134

   Purpose

   Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

   Depositor

   Joseph Sorg

   Article

   McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

   Inserts

   Upstream & Downstream pyrE deletion region
   tetR
   Cas9
   gRNA
   

   Use

   E. coli - c. difficile shuttle vector

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-PM

   Plasmid

   #197386

   Purpose

   To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM808

   Plasmid

   #134181

   Purpose

   Lentiviral sgRNA plasmid with blasticidin resistance

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64-GFP-ePDZ-CAAX

   Plasmid

   #135603

   Purpose

   Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   Lifeact-GFP-ePDZ-CAAX
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   CAAX, EGFP, and ePDZ

   Promoter

   Unknown

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPLtetO-FtsA

   Plasmid

   #107558

   Purpose

   Plasmid for titrating synthesis of FtsA with doxycycline

   Depositor

   Uwe Sauer

   Article

   Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

   Insert

   ftsA
   

   Use

   Synthetic Biology

   Promoter

   pLtetO

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSAR-Cre-F2A-I-SceI

   Plasmid

   #62302

   Purpose

   Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Insert

   Cre recombinase, I-SceI nuclease
   

   Expression

   Insect

   Promoter

   vasa

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE1108

   Plasmid

   #202772

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUB-cHyPer2

    Plasmid

    #84728

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2
    

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPLtetO-FtsZ

    Plasmid

    #89656

    Purpose

    Plasmid for titrating synthesis of FtsZ with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    GTP-binding tubulin-like cell division protein
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pV1F

    Plasmid

    #205084

    Purpose

    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

    Depositor

    Suomeng Dong

    Article

    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

    Insert

    AtVIP1
    

    Expression

    Bacterial

    Promoter

    agorbacterium virB operon promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBA328

    Plasmid

    #214748

    Purpose

    Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RBS(22821)-mcherry
    

    Expression

    Bacterial

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Aurli_150841_GZG

    Plasmid

    #162563

    Purpose

    Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

    Depositor

    Jackie Collier

    Article

    Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

    Insert

    shble
    

    Use

    Unspecified

    Promoter

    GAPDH

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMARO

    Plasmid

    #178086

    Purpose

    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Insert

    TcR (tetA/tetR)
    

    Expression

    Bacterial

    Mutation

    Tetracycline resistance cassette is flanked by Ec…

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPLtetO-ClpX

    Plasmid

    #89657

    Purpose

    Plasmid for titrating synthesis of ClpX with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    clpX
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDE43-MCK

    Plasmid

    #49523

    Purpose

    Kanamycin and Chloramphenicol resistant; destination vector engineered to integrate into the mycobacteriophage att-L5 site and designed to be used with the multisite Gateway cloning system

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7C-HSPA1L

    Plasmid

    #177660

    Purpose

    Recombinant protein synthesis of human HSPA1L

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSPA1L (HSPA1L Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTX1-SMN1-exon7-ESE1

    Plasmid

    #126041

    Purpose

    In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN1 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCORE-Kp53

    Plasmid

    #72241

    Purpose

    Contains the CORE cassette with KanMX4 and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    kanMX4
    

    Expression

    Bacterial

    Mutation

    V122A mutation

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCS2xVlk1-3xFlag

    Plasmid

    #172773

    Purpose

    mRNA synthesis for injection into embryos

    Depositor

    Edward De Robertis

    Article

    Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

    Insert

    Vlk1 (pkdcc.1.S Frog)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA-flag-mKdm4d(H189A)-polyA

    Plasmid

    #61554

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (catalytic mutant)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    changed Histidine 189 (of Kdm4d) to Alanine

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pWJB30

    Plasmid

    #126505

    Purpose

    Salmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30

    Depositor

    Ute Romling

    Article

    Simm et al Mol Microbiol. 2004 Aug;53(4):1123-34. d

    Insert

    adrA-8xHis
    

    Tags

    8xHis

    Expression

    Bacterial

    Promoter

    PBAD30

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRecLV103-GFP-PGBD5

    Plasmid

    #65409

    Purpose

    Lentiviral expression vector for fusion of GFP with human PGBD5

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUG-UBC8

    Plasmid

    #104673

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    AtUBC8 (UBC8 Mustard Weed)
    

    Use

    Synthetic Biology

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pETCON-TNF

    Plasmid

    #67748

    Purpose

    Tumor Necrosis Factor Alpha Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    TNF-Alpha
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Expression

    Bacterial

    Mutation

    V122A mutation

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFGC5941-PacI-ath-STTM2937

    Plasmid

    #84112

    Purpose

    target miRNA2937 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM2937
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFGC5941-PacI-ath-STTM159

    Plasmid

    #84084

    Purpose

    target miRNA159 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM159
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFGC5941DR5-PacI-ath-STTM390

    Plasmid

    #84117

    Purpose

    target miRNA390 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM390
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFGC5941-PacI-ath-STTM858a/b

    Plasmid

    #84177

    Purpose

    target miRNA858 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM858a/b
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFGC5941-PacI-ath-STTM828

    Plasmid

    #84157

    Purpose

    target miRNA828 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM828
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

35. pFGC5941-PacI-ath-STTM843

    Plasmid

    #84169

    Purpose

    target miRNA843 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM843
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFGC5941-PacI-ath-STTM845

    Plasmid

    #84170

    Purpose

    target miRNA845 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM845
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. UG75-ADH1-DGK1-3HA

    Plasmid

    #198595

    Purpose

    DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    DGK1 (DGK1 Budding Yeast)
    

    Tags

    3HA

    Expression

    Yeast

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. UG75-ADH1-PAH1-3HA

    Plasmid

    #198597

    Purpose

    PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PAH1 (PAH1 Budding Yeast)
    

    Tags

    3HA

    Expression

    Yeast

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFGC5941-PacI-ath-STTM173

    Plasmid

    #84101

    Purpose

    target miRNA173 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM173
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge