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Showing: 3951 - 4000 of 7988 results

  9. GPR62-DuET

    Plasmid
    #213288
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR62 (GPR62 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213288-plasmid-map-sequence-id-430833

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  10. TFORF0700

    Plasmid
    #143725
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PAXBP1 (PAXBP1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143725-plasmid-map-sequence-id-407887

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  12. TFORF2867

    Plasmid
    #142163
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TADA3 (TADA3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142163-plasmid-map-sequence-id-397697

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  13. TFORF2868

    Plasmid
    #142164
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TADA3 (TADA3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142164-plasmid-map-sequence-id-397676

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  14. TFORF0225

    Plasmid
    #142365
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF593 (ZNF593 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142365-plasmid-map-sequence-id-397183

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  15. IR76b-T2A-QF2

    Plasmid
    #162523
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162523-plasmid-map-sequence-id-354669

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  16. GR3-T2A-QF2

    Plasmid
    #162521
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162521-plasmid-map-sequence-id-372762

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  17. IR8a-T2A-QF2

    Plasmid
    #162520
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162520-plasmid-map-sequence-id-372758

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  19. TFORF0226

    Plasmid
    #141557
    Purpose
    Lentiviral vector for overexpressing the ZNF593 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF593 (ZNF593 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141557-plasmid-map-sequence-id-276807

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  24. IR25a-T2A-QF2

    Plasmid
    #162522
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162522-plasmid-map-sequence-id-354640

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  28. pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355

    Plasmid
    #105638
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino acids 328-355) from bicistronic plasmid
    Depositor
    Thanos Halazonetis
    Inserts
    Rev7 (Mad2l2 Mouse)
    Champ1 (CHAMP1 Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105638-plasmid-map-sequence-id-203917

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  30. pJV126

    Plasmid
    #124250
    Purpose
    In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124250-plasmid-map-sequence-id-242199

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  31. pJV127

    Plasmid
    #124251
    Purpose
    In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124251-plasmid-map-sequence-id-242078

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  32. pJV128

    Plasmid
    #124252
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124252-plasmid-map-sequence-id-242223

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  33. pJV129

    Plasmid
    #124253
    Purpose
    In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.
    Depositor
    Stanley Riddell
    Article
    Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92
    Insert
    Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
    Use
    In vitro transcription template
    Promoter
    T7
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    124253-plasmid-map-sequence-id-242081

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Showing: 3951 - 4000 of 7988 results