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MSCV-NSD3-short del(280-342)

Plasmid

#72550

Purpose

expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

280-342 aa deletion

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-497/miR-195

Plasmid

#64236

Purpose

Expresses miR-497 and miR-195 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-497 and miR-195 (MIR195, MIR497 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Expression

Mutation

mutant KRAS4A-G12V

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(384-645)

Plasmid

#72549

Purpose

expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

384-645 aa deletion

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_BRG1BD-PGK-Puro-IRES-GFP

Plasmid

#75121

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRG1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

Bromodomain replaced with the bromodomain of BRG1

Promoter

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BRD1BD-PGK-Puro-IRES-GFP

Plasmid

#75119

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD1 bromodomain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Tags

Expression

Mutation

Bromodomain replaced with the bromodomain of BRD1

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-GFP

Plasmid

#75130

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
MSCV T274V S392A KSR1 IRES GFP

Plasmid

#25979

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine and serine 392 to…

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
MSCV-PIG-FLAG-TAF4(827-909)

Plasmid

#105617

Purpose

retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode83

Plasmid

#83760

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode83

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(100-263)

Plasmid

#72548

Purpose

expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3xFlag and SV40 NLS

Expression

Mammalian

Mutation

100-263 aa deletion

Promoter

LTR

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-STIM2 WPRE

Plasmid

#236236

Purpose

AAV expression of human STIM2 internally tagged with mScarlet-I

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI-STIM2 (STIM2 Human)

Use

AAV

Tags

mScarletI in position 29

Expression

Mutation

Signal peptide from STIM1 and residues 15-746 of …

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING L373A with IRSp53 pLxIS

Plasmid

#221283

Purpose

Expression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

fusion of FKBP-STING (L373A) with IRSp53 pLxIS (RLL361NLV) (Sting1, Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-miR122 TS

Plasmid

#209783

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter, incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

miR122 ts

Use

AAV

Tags

HA

Expression

Mutation

Promoter

cTNT

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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p186-RV-CAG-dio-mScarlet-T2A-mVenus

Plasmid

#204740

Purpose

Conditional expression of target gene mVenus with fluorescent reporter mScarlet

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mVenus

Use

Retroviral

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

Plasmid

#172435

Purpose

Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV puro PRDM14 ΔZinc finger domain

Plasmid

#193679

Purpose

Constitutive retroviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Retroviral

Tags

Expression

Mutation

Deletion of Zinc finger domain

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-neo C-HAonly HPV16 E7 E80A/D81A

Plasmid

#191541

Purpose

Protein expression of HPV16 E7 E80A/D81A

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16 E7

Use

Retroviral

Tags

HA

Expression

Mutation

E80A, D81A

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-mouse-Caspase-1-H340D

Plasmid

#183362

Purpose

Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 H340D (Casp1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

H340D

Promoter

MESV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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