We narrowed to 9,182 results for: Pol;

1. pET11-PC(75-228)-H6

   Plasmid

   #183779

   Purpose

   Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-SU(Z)12(624-900)-H6

   Plasmid

   #183772

   Purpose

   Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

   Insert

   SU(Z)12 residues 624-900 (Su(z)12 Fly)
   

   Tags

   His tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. L1-Ck1-hs1-l-chlo

   Plasmid

   #163937

   Purpose

   For integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha trnG_trnfM region left homologous sequence
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. L1-Ck4-hs1-r-chlo

   Plasmid

   #163940

   Purpose

   For integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha trnG_trnfM region right homologous sequence
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. L1-Ck4-hs2-r-chlo

   Plasmid

   #163941

   Purpose

   For integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha rbcL_trnR region right homologous sequence
   

   Expression

   Bacterial

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV5endoNA2

   Plasmid

   #174729

   Purpose

   Expresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

   Insert

   V5 epitope tag
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNOC-CRISPR-compact

   Plasmid

   #99369

   Purpose

   Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   Cas9-Nlux

   Promoter

   Ribi promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPK1E-2

   Plasmid

   #48146

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPK1E-1

    Plasmid

    #48145

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. D-raf G621S

    Plasmid

    #39769

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    G621S

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

12. D-raf C295S

    Plasmid

    #39768

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    C295S

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

13. D-raf F290I

    Plasmid

    #39766

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    F290I

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

14. D-raf R217L+S620A

    Plasmid

    #39765

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    R217L and S620A

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

15. D-raf R217L+C295S

    Plasmid

    #39764

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    R217L and C295S

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pET-28a_6H-TAQ_E602D

    Plasmid

    #166944

    Purpose

    Bacterial expression of Taq DNA polymerase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    Thermus aquaticus DNA polymerase
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    E602D

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. eDrIR1

    Plasmid

    #183998

    Purpose

    Optically controlled eDrIR1 expression in mammalian cells

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrIR1 (INSR Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB_TRE3G_scFV-sfGFP-Kmt5cFL

    Plasmid

    #235581

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    GPDp

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCCM’

    Plasmid

    #162709

    Purpose

    pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    Second CCM operon cloned from H. neapolitanus
    

    Expression

    Bacterial

    Mutation

    p15A origin replaced with high-copy colE1 origin

    Promoter

    PLtet0-1 promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEAK-Flag/INPP4B

    Plasmid

    #24324

    Depositor

    Lewis Cantley

    Article

    Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

    Insert

    Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    none

    Available Since

    May 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. PRE-Hsp70BbCas9_1.3

    Plasmid

    #190797

    Purpose

    aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Expression

    Insect

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-Syn-Ace-mNeon2

    Plasmid

    #195518

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    Synapsin

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Syn-VARNAM2-Kv2.1PR

    Plasmid

    #195524

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRcCMV myc-Plk1 wt (Nigg RG6)

    Plasmid

    #41160

    Depositor

    Erich Nigg

    Article

    Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

    Insert

    Plk1 (PLK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCAG mEmerald-LAMP1

    Plasmid

    #168513

    Purpose

    Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

    Plasmid

    #235590

    Purpose

    Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Mutation

    NHDC182-185AAAG

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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