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Addgene

We narrowed to 18,864 results for: IRE

Showing: 3201 - 3240 of 18864 results
  1. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. RNA-Protein Landing Pad

    Plasmid
    #226162
    Purpose
    Used to generate a landing pad in Flp-In cells containing promoters for protein and MS2-circRNA barcode expression
    Depositor
    Inserts
    mTagBFP2-P2A-iCasp9
    Reverse tetracycline transactivator 3
    Hygromycin-IRES-EGFP
    Use
    Synthetic Biology
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. p1D4-hrGFP II

    Plasmid
    #71317
    Purpose
    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    1D4 epitope (TETSQVAPA) and IRES-hrGFPII
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. mscv2.2-NAIP5

    Plasmid
    #60205
    Purpose
    Mouse NAIP5 (B6 allele), expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of NAIP5
    Depositor
    Insert
    NAIP5
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pCAG-HDAC4-IGd2

    Plasmid
    #22536
    Purpose
    To be used for the RNAi screening purposes (not for HDAC4 expression); see paper
    Depositor
    Insert
    Histone Deacetylase 4 (HDAC4 Human)
    Use
    Tags
    IGd2 = Ires-destabilized GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2009
    Availability
    Academic Institutions and Nonprofits only
  7. pATF1-donor

    Plasmid
    #112328
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP
    Depositor
    Insert
    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. cytoplasmic NAD+ biosensor

    Plasmid
    #186787
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm
    Depositor
    Insert
    cytoplasmic NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. nuclear NAD+ biosensor

    Plasmid
    #186789
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus
    Depositor
    Insert
    nuclear NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pCMV-Spike-VLP

    Plasmid
    #173922
    Purpose
    Expresses virus-like particles based upon the original SARS-Cov-2
    Depositor
    Inserts
    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
    Use
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3201 - 3240 of 18864 results