We narrowed to 23,099 results for: CRISPR
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Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Modular BE4max for GG insertion
Plasmid#198891PurposeEasily insert deaminase of choice into BE4max using Golden Gate cloningDepositorInsertCRISPR associated protein 9 (cas9 Streptococcus pyogenes)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_TypeIC_acrIF2_invivo
Plasmid#196402Purposeexpresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assayDepositorInsertanti-CRISPR IF2 protein (orf30 P. aeruginosa)
UseTagsExpressionBacterialMutationPromoterT7Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_TypeIC_acrIC4_invivo
Plasmid#196403Purposeexpresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assayDepositorInsertanti-CRISPR IC4 protein
UseTagsExpressionBacterialMutationPromoterT7Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-kan
Plasmid#184836PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK14 pCAS-Tyr-[gRNA: 4=XII-5] (SplitKanR, AmpR)
Plasmid#178998PurposeSp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK13 pCAS-Tyr-[gRNA: 3=XII-2] (SplitKanR, AmpR)
Plasmid#178997PurposeSp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEL086
Plasmid#137894PurposeiSTU-CRISPR/Cas9 with RcCat intron, RZ structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL077
Plasmid#137886PurposeiSTU-CRISPR/Cas9 with StIV2 intron, NU structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL079
Plasmid#137888PurposeiSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL078
Plasmid#137887PurposeiSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0+N497A+Q695A+Q926A
Plasmid#108300PurposepX459 V2.0 (Plasmid #62988) with the N497A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable SinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
px330-MRPsg3
Plasmid#87191Purposedisruption of human RMRP gene via CRISPR-Cas9, guide 3DepositorInserthuman MRP RNA CRISPR guide #3 (RMRP Human)
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianMutationPromoterMSCV LTR promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHSN6I01
Plasmid#50587PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 that is defective in DNA cleavage; the maiz…Promoter2×35Sp and AtU6-26pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHSN6A01
Plasmid#50586PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…Promoter2×35Sp and AtU6-26pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA1
Plasmid#113130PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 for gRNA expression, RSV for GFP expressionAvailable SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBUN6A11
Plasmid#50579PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…PromoterOsU3p and Ubi1pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBUN6I11
Plasmid#50580PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 that is defective in DNA cleavage; the maiz…PromoterOsU3p and Ubi1pAvailable SinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only