We narrowed to 5,818 results for: pcas

1. pCAG-mEGFP-CaMKIIa (T286D)

   Plasmid

   #127391

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Aspartic acid

   Promoter

   pCAG

   Available Since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SspB-mScarlet-I-MYPT169

   Plasmid

   #178522

   Purpose

   Expresses SspB-mScarlet-I-MYPT (1-169 aa) in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   SspB-mScarlet-I-MYPT169
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-hGPR56-IRES-GFP

   Plasmid

   #52297

   Purpose

   Expresses human GPR56 and GFP in mammalian cells.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Human GPR56 coding sequence (ADGRG1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-tomato SOX2-sgRNA

   Plasmid

   #196190

   Purpose

   Editing human SOX2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2 (SOX2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9 nickase for Split-PE (pJUL2497)

   Plasmid

   #190104

   Purpose

   Expresses nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Tags

   XTEN-bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS Donor mClover-LMNA

   Plasmid

   #98970

   Purpose

   Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-HA-rat Nrxn3beta AS4(-)

   Plasmid

   #58269

   Purpose

   Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

   Depositor

   Peter Scheiffele

   Article

   Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

   Insert

   Nrxn3beta AS4(-) (Nrxn3 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-AsCas12a-Plus-mCherry

   Plasmid

   #191231

   Purpose

   To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   AsCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Mutation

   E174R, R951K, R955A

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Kir2.1Mut-T2A-tdTomato

    Plasmid

    #60644

    Purpose

    Expresses Kir2.1Mut-T2A-tdTomato

    Depositor

    Massimo Scanziani

    Article

    Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

    Insert

    Kcnj2 (Kcnj2 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GYG to AAA (aa144-146)

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-YE1-BE4max

    Plasmid

    #198549

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS SARS-CoV-2 BA.1 Spike

    Plasmid

    #185452

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.1 Spike

    Depositor

    Marceline Côté

    Article

    Chatterjee et al Cell Rep. 2022 Mar 1;38(9):110429. doi:

    Insert

    SARS-CoV-2 Spike BA.1 (S )
    

    Expression

    Mammalian

    Mutation

    Contains the following mutations: A67V, Δ69-70, T…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. eSpCas9(1.1)-T2A-Puro

    Plasmid

    #101039

    Purpose

    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

    Depositor

    Andrea Németh

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFLAG and T2A-Puro

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-3xFLAG-BioID2-MCS

    Plasmid

    #186811

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (N terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Tags

    3xFLAG-BioID2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-MCP-SRSF1-V5-mCherry

    Plasmid

    #235088

    Purpose

    SRSF1 mCherry fusion protein with MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Sox2-IRES-Neo

    Plasmid

    #13462

    Depositor

    Shinya Yamanaka

    Article

    Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

    Insert

    Sox2 (Sox2 Mouse)
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-GBP1-10gly-lexADBD

    Plasmid

    #50795

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-lexA DNA binding domain fusion protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid

    #100540

    Purpose

    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa), Y276H muta…

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-mTagBFP2-T2A-FLPo

    Plasmid

    #124834

    Purpose

    Monomeric TagBFP2 and FLP recombinase

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-FLPo
    

    Expression

    Mammalian

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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