168,189 results
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Plasmid#195949PurposeGateway p5E vectorDepositorInsertexorh regulatory elements
UseOtherAvailable SinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-NEUROD1
Plasmid#215031PurposeMammalian expression of human NEUROD1DepositorAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110
Plasmid#135480PurposeE. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-HEK3_CTT_ins
Plasmid#132778PurposeS. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editingDepositorInsertHEK3_CTT_ins pegRNA
ExpressionMammalianMutationSee manuscriptPromoterU6Available SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_7
Plasmid#60578Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_7
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-PE2-His (IK1822)
Plasmid#170103PurposeT7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLSDepositorInsertbpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
UseCRISPRTags6xHis tagExpressionBacterialMutationBacteria codon-optimized nSpCas9 (H840A) & M-…PromoterT7Available SinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pS-sg:GFP
Plasmid#196296Purpose35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSsDepositorInsertFull length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNb-nALFA
Plasmid#159988PurposeFor the protein expression with ALFA tag at N-terminus in E. coliDepositorTypeEmpty backboneTagsMVKYI-8histidine-ALFA tag-HRV3C protease cleavage…ExpressionBacterialAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-FLEX-BFP2-(mCherry)’
Plasmid#115234PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
ExpressionMammalianAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only